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A molecular marker of adpgk gene related to the testis traits of Hu sheep and its application

A molecular marker and molecular marker-assisted technology, applied in the field of molecular biology, can solve the problems that have not yet been reported, and achieve the effects of high selection efficiency, accelerated breeding process, and clear selection goals.

Active Publication Date: 2022-04-29
甘肃润牧生物工程有限责任公司
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Problems solved by technology

However, at present, the application of ADPGK is mainly concentrated in tumor cells, and the relevant research on the fertility of Hu sheep and even sheep has not been reported yet.

Method used

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  • A molecular marker of adpgk gene related to the testis traits of Hu sheep and its application
  • A molecular marker of adpgk gene related to the testis traits of Hu sheep and its application
  • A molecular marker of adpgk gene related to the testis traits of Hu sheep and its application

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Embodiment Construction

[0016] The following embodiments are further descriptions of the present invention, but should not be construed as limiting the present invention. On the premise of not departing from the spirit and essence of the present invention, any modifications or replacements made to the present invention shall fall within the protection scope of the present invention.

[0017] The 6-month-old Hu sheep ram sample of the present invention was collected from Minqin County Defu Agricultural Biotechnology Co., Ltd.

[0018] 1. Hu sheep testis tissue and epididymis tail semen collection

[0019] The left testis tissue of the above-mentioned Hu sheep ram was taken, stored in liquid nitrogen, and used for RNA extraction. Weigh the left epididymis tail of Hu sheep and soak it in 50 mL saline, cut the tissue into small pieces with sterile scissors and put them in a test tube, incubate in a water bath at 37°C for 1 hour, and filter through a 200-mesh copper mesh to remove Tissue fragments, seme...

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Abstract

The invention discloses a method related to the testicular traits of Hu sheep. ADPGK Gene molecular markers and applications thereof, the molecular markers are composed of ADPGK The gene sequence (Gene Bank ID: 101116836) was amplified by the designed specific primers. Its nucleotide sequence is shown in the sequence table SEQ ID NO.1, and there is a C at the 130th bp of the sequence table SEQ ID NO.1 / T base mutation, which leads to HpyCH4 Ⅲ‑RFLP restriction polymorphism. By analyzing the Hu sheep ADPGK The correlation between genotyping and testicular traits, the results show that the genotypes of Hu sheep individuals are divided into CC genotype and TC genotype, and the testis weight, size, epididymis weight and sperm count of TC genotype individuals are significantly higher than those of CC genotype individuals . The molecular marker of the present invention improves the accuracy of seed selection, and can select Hu sheep individuals with higher sperm production when Hu sheep rams are young, making early breeding of Hu sheep varieties possible and accelerating the breeding process.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a method related to the testicular traits of Hu sheep. ADPGK Gene molecular markers and their applications. Background technique [0002] In recent years, as the demand for mutton continues to increase, the supply of mutton is in short supply. Therefore, cultivating new breeds or strains of sheep with high fecundity is one of the main measures to alleviate the tension of mutton in my country, and it is also one of the important tasks of the joint research of national livestock and poultry breeding. Sheep artificial insemination technology is the best choice for intensive sheep farms to rapidly expand the breeding scale, carry out scientific selection and matching, and improve breeding efficiency. Therefore, increasing the sperm production of rams can effectively increase the production capacity of the flock and improve the current situation of insufficient...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 李万宏要荣宇路婷婷刘佳美乐祥鹏翁秀秀李发弟
Owner 甘肃润牧生物工程有限责任公司
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