Judgment method for viability of juvenile eriocheir sinensis
A technology for Chinese mitten crab and juvenile crab, applied in fish farming, climate change adaptation, animal husbandry, etc., can solve the problem of difficulty in judging the vitality of juvenile crabs, affecting the quality of adult crab products, and wasting breeding resources from juvenile crabs to adult crabs. and other problems, to achieve good reliability, improve product quality, and save breeding resources.
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Embodiment 1
[0028] The establishment of the viability judging method of embodiment 1 Chinese mitten crab juvenile crab
[0029] Step 1: Sampling young crab seedlings, and measuring parameters related to vitality intensity (tissue supernatant osmotic pressure, ion concentration, non-specific immune enzyme activity) based on different salinity water bodies.
[0030] Step (1): Randomly sample the juvenile crabs of the same batch, and the sampling ratio is 1‰-3‰ of the weight of each batch of juvenile crabs. Specifically, 900 juvenile crabs with neat specifications, no body trauma, and healthy limbs were selected from the Chongming base of Shanghai Ocean University.
[0031] Step (2): use bittern and tap water after aeration, and adopt the step-by-step salt-increasing method to prepare water bodies with salinity of 8‰, 16‰, and 32‰.
[0032] Step (3): Set up 3 parallel groups for each salinity-stressed water body. In step (1), sample young crabs and put them into each group of water bodies e...
Embodiment 2
[0052]In March 2019, the study on the osmotic regulation and immunity of Chinese mitten crab juveniles under acute salinity stress was carried out at the experimental base of Shanghai Ocean University. Three cultivation ponds with neat specifications, no physical trauma, and mitten crabs were selected from the Chongming base of Shanghai Ocean University. Each 1000 juvenile crabs with sound limbs, after temporary support, utilize the method of embodiment 1 to carry out viability judgment to the juvenile crabs of Chinese mitten crab, specifically:
[0053] (1) Randomly sample 100 juvenile crabs with neat specifications, no body trauma, and healthy limbs in 3 breeding ponds at the Chongming base of Shanghai Ocean University.
[0054] (2) Using salt bittern and tap water after aeration, the water body with salinity of 0‰, 8‰, 16‰, and 32‰ was prepared by gradually increasing salt method.
[0055] (3) Set up three parallel groups for each salinity-stressed water body. Step (1) Samp...
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