Serum-free medium for mesenchymal stem cells

Abstract

The invention belongs to the field of biological medicines and relates to a serum-free medium for mesenchymal stem cells. The serum-free medium provided by the invention comprises a basic medium and additive components, wherein the additive components comprise a JNK pathway inhibitor, an ERK1 / 2 pathway inhibitor, a P38MAPK pathway inhibitor and aminoethanol; the JNK pathway inhibitor comprises but is not limited to SP600125; the ERK1 / 2 pathway inhibitor comprises but is not limited to PD0325901; and the P38MAPK pathway inhibitor comprises at least one of SB202190, SB203580 and BIRB796. The medium for mesenchymal stem cells disclosed by the invention does not contain FBS and does not contain any heterologous animal-derived component, the probability of cell contamination is reduced, and an effect of promoting MSC amplification is achieved.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a serum-free medium for mesenchymal stem cells. Background technique [0002] Stem cells can be divided into totipotent stem cells, pluripotent stem cells, progenitor cells, etc. according to different differentiation capabilities, and can be divided into placental stem cells, adipose stem cells, hematopoietic stem cells, etc. according to different sources. Mesenchymal stem cells (MSC, mesenchymal stem cells) are an important member of the stem cell family, derived from the mesoderm in the early development stage, and belong to pluripotent stem cells. MSC was originally found in the bone marrow because of its multi-directional differentiation potential, hematopoietic support and promotion Stem cell implantation, immune regulation and self-replication have attracted increasing attention. For example, mesenchymal stem cells can be differentiated into fat, bone, cartilage, muscle, tendon, ...

AI Technical Summary

Problems solved by technology

[0003] Although the current animal experiments and some clinical studies have found that MSCs have a good therapeutic effect on promoting tissue repair and regulating abnormal immune responses, there are still many problems in the application of MSCs that have not been resolved: firstly, large-scale expansion in vitro is the prerequisite for clinical treatment of MSCs , but there is no agreement on the in vitro expansion system of MSC: according to the different types of culture medium, the MSC expansion system can be divided into a culture system containing fetal bovine serum and a culture system with defined components, no animal-derived components, and no serum. Training system

[0004] The culture system based on fetal bovine serum has been proven to be the most effective culture system for MSC cells. Under this system, MSC cells have excellent proliferation and differentiation capabilities, and have good potential for clinical treatment. However, the main problems of fetal bovine serum There is no specific index to evaluate the quality of serum, so the quality of fetal bovine serum varies from place to place and between batches, and the quality of cultured cells will also vary. Studies have confirmed that MSC cells will appear "aging" in advance when inferior serum is used to cultivate MSC cells. "Phenomenon, the appearance of cells becomes non-spindle-shaped, telomerase gradually shortens, cell proliferation ability decreases, and the differentiation potential of cells into bone cells, chondrocytes, and adipocytes is also significantly weakened.

Therefore, more and more researchers are currently cultivating MSC cells with a culture system with defined components, no animal-derived components, and no serum. From the current results, it can be seen that the serum-free system can support long-term culture of MSC cells, but it is different from fetal bovine serum. There are still deficiencies compared with the system, the main problem is that the cell proliferation is slow and the differentiation ability is different

At present, the most ideal serum substitute is human platelet lysate, which can not only avoid the possibility of heterogeneous virus contamination brought by fetal bovine serum, but also completely replace serum to promote the in vitro culture of MSC, but the source of platelet lysate is currently existing. The main problem, the high price greatly limits its application scale

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