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Lactobacillus acidilactici, aquatic feed additive, fish feed and application of lactobacillus acidilactici, aquatic feed additive and fish feed

An aquaculture feed, Lactobacillus technology, applied in Lactobacillus, animal feed, animal feed and other directions, can solve the problems of poor aquaculture effect, unstable effect, etc., to improve the ability to resist Edwardsiella infection, improve immunity, The effect of reducing morbidity

Active Publication Date: 2021-09-17
CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of Lactobacillus sourfish, aquatic feed additive and fish feed and their uses in order to overcome the existing lactic acid bacteria additives in the prior art that are used in aquaculture with poor or unstable effects.

Method used

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  • Lactobacillus acidilactici, aquatic feed additive, fish feed and application of lactobacillus acidilactici, aquatic feed additive and fish feed
  • Lactobacillus acidilactici, aquatic feed additive, fish feed and application of lactobacillus acidilactici, aquatic feed additive and fish feed
  • Lactobacillus acidilactici, aquatic feed additive, fish feed and application of lactobacillus acidilactici, aquatic feed additive and fish feed

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Embodiment approach

[0046] According to a particularly preferred embodiment of the present invention, the formula of the fish feed can be:

[0047]

[0048]

[0049] Preferably, the vitamins in the fish feed are added with a vitamin premix, and the vitamin premix preferably contains vitamin A, vitamin D3, vitamin K, vitamin E, vitamin B1, vitamin B2, vitamin 6, and vitamin B12 , vitamin H, vitamin C, calcium pantothenate, niacin, folic acid and at least one of choline chloride. The vitamin premix can be a commercially available related product, or a related product prepared by yourself according to actual needs. Those skilled in the art can adjust the components and the dosage of each component in the vitamin premix according to the actual situation (such as fish growth needs, breeding environment conditions, etc.), and details will not be repeated here.

[0050] Preferably, the minerals in the fish feed are added with a mineral premix, and the mineral premix preferably contains at least o...

Embodiment 1

[0075] This example is used to illustrate the isolation, identification and preservation of Lactobacillus acidicus provided by the present invention.

[0076] Strain P1 was isolated from intestinal chyme of channel catfish and identified as Lactobacillus acidipiscis (La) by 16S rDNA sequencing.

[0077] On February 02, 2021, the strain P1 was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures (Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, abbreviation of the depository unit: CGMCC), and the deposit number is CGMCCNo. 21806.

Embodiment 2

[0079] This example is used to illustrate the inhibitory effect of Lactobacillus acidfish provided by the present invention on Aeromonas hydrophila and Vibrio parahaemolyticus in vitro experiments.

[0080] The MRS medium used in this example was purchased from Oxoid Company in the United Kingdom, the article number is CM1163; the nutrient broth medium was purchased from Hangzhou Best Biotechnology Co., Ltd., the article number was BS1003; the nutrient agar medium was purchased from Beijing Aoboxing Biotechnology Co., Ltd. Company, Cat. No. 02-276.

[0081] The bacterial strain P1 (i.e. Lactobacillus acidfishus CGMCC No.21806) isolated in Example 1 and Enterococcus faecium (CICC20430), a commonly used strain of lactic acid bacteria, were cultured at 37° C. for 72 hours using MRS medium, and then the concentration of 2×10 9 Cells / mL of bacterial solution 1 (P1) and bacterial solution 2 (Enterococcus faecium) were used for later use.

[0082] Vibrio parahaemolyticus and Aeromo...

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Abstract

The invention relates to the field of aquaculture, and discloses lactobacillus acidilactici, an aquatic feed additive based on the lactobacillus acidilactici, fish feed and application of the lactobacillus acidilactici, the aquatic feed additive and the fish feed. The lactobacillus acidilactici can promote the growth performance of cultured fishes, improve the immunity of the cultured fishes, especially improve the capacity of resisting Edwardsiella infection of longsnout catfish, well adapt to the digestive tract environment of the cultured fishes and stably play a role.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to Lactobacillus acidicus, an aquatic feed additive, fish feed and applications thereof. Background technique [0002] my country is a big aquaculture country. In 2019, the total aquaculture output was 50.7907 million tons, accounting for more than 60% of the world's total aquaculture output. Among them, the total output of fish reached 27.086 million tons, accounting for more than 53% of the total output of aquaculture. Due to the continuous increase of breeding density and the continuous improvement of the level of breeding intensification, the prevalence of diseases and the safety of aquatic products have become important factors restricting the healthy development of aquaculture. [0003] Disinfectants for external use and oral administration of antibiotics are currently the main means of treating bacterial diseases in aquaculture animals. However, the long-term use of antibiotics in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K50/80A23K10/18C12R1/225
CPCC12N1/20A23K50/80A23K10/18A23V2400/11Y02A40/818
Inventor 张洪玉夏磊穆迎春宋金龙王海波
Owner CHINESE ACAD OF FISHERY SCI
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