High-pressure-resistant liquid chromatography connector and fluid type connection system achieving zero dead volume

Abstract

The invention discloses a high-pressure-resistant liquid chromatography connector and a fluid type connecting system for realizing zero dead volume. The high-pressure-resistant liquid chromatogram connector comprises an assembly nut including an outer thread and a tight breaking end face, and an axial hole I is arranged in the assembly nut in a penetrating mode; a hoop including a pressing end face and an extending section, wherein the tight breaking end face abuts against the pressing end face, and an axial hole II allowing a pk pipe to penetrate through is arranged in the hoop in a penetrating mode; and a peek pipe including a pipe body and a bearing end, an axial hole III allowing a capillary tube to penetrate through is arranged in the peek pipe in a penetrating mode, the pipe body is embedded into the axial hole II, and one end of the pipe body extends into the axial hole I. According to the technical scheme, the high-pressure-resistant liquid chromatography connector is high in adaptability and can be matched with an existing 10-32-thread 1 / 16 connector on the market.

Description

technical field [0001] The invention relates to the technical field of chromatography, in particular to a high-pressure liquid chromatography joint and a fluid connection system for realizing zero dead volume. Background technique [0002] High-performance liquid chromatography (HPLC) is an efficient and rapid analysis and separation technology developed in the 1970s, and is an important means of modern biochemical analysis. The basic separation principle of chromatography is: when the components dissolved in the mobile phase (mobile phase) pass through the stationary phase, due to the size and strength of the interaction (adsorption, distribution, exclusion, affinity) with the stationary phase (station phase), Weakness is different, and the retention time in the stationary phase is different, so they flow out of the stationary phase successively. [0003] HPLC is developed on the basis of classic liquid chromatography, using liquid as the mobile phase, and adopts column ch...

AI Technical Summary

Problems solved by technology

In addition, for the liquid phase with nanoliter flow rate, the dead volume at the joint will seriously affect the peak time and reduce the separation efficiency of the liquid phase, so the liquid phase joint with zero dead volume is an indispensable part of nanoliter liquid chromatography

[0006] The common high-pressure resistant 1 / 16″ chromatographic joints on the market generally integrate three parts together, and are squeezed and sealed by the peek tube at the front end of the joint. Due to the frequent extrusion of the pipe, the sealing pipe of the peek material is worn, which in turn leads to damage to the joint.

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