Mesenchymal stem cell source exosome based on Tongxinluo pretreatment and preparation method thereof

A technique for stem cells and exosomes, which is applied in the field of high-efficiency exosomes derived from mesenchymal stem cells and their preparation, can solve the problems of insignificant effects and the like

Active Publication Date: 2021-09-10
FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The paracrine function of MSCs is an important way for them to function (Ranganath, S.H., et al., Harnessing the mesenchymalstem cell secretome for the treatment of cardiovascular disease. Cell StemCell, 2012.1...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mesenchymal stem cell source exosome based on Tongxinluo pretreatment and preparation method thereof
  • Mesenchymal stem cell source exosome based on Tongxinluo pretreatment and preparation method thereof
  • Mesenchymal stem cell source exosome based on Tongxinluo pretreatment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation method of exosomes derived from mesenchymal stem cells:

[0034] The preparation method of exosomes derived from mesenchymal stem cells: the primary rat (Sprague-Dawley rat, 60-80g) BM-MSCs were isolated by the differential attachment method, and the BM-MSCs were subcultured and expanded to the 3rd-4th generation for later use. BM-MSCs complete medium (IMDM, Gibco, USA) was pretreated with 400 μg / mL Tongxinluo for 24 hours, and the cell medium was replaced with IMDM partial medium (without adding fetal bovine serum) to continue culturing. After 48 hours, the conditioned medium was collected and ultracentrifugation was used to separate the exosomes secreted by BM-MSCs pretreated with Tongxinluo (MSC TXL -Exo). The specific steps of the ultracentrifugation method include: after collecting the conditioned medium, centrifuge at 300g for 10min to remove cells; centrifuge at 2000g for 20min to remove cell debris; high-speed centrifuge at 16500g for 30min to remov...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a mesenchymal stem cell source exosome based on Tongxinluo pretreatment and a preparation method thereof. The invention provides the preparation method of the mesenchymal stem cell source exosome for up-regulating the expression level of miR-146a. The method comprises the following steps of pretreating mesenchymal stem cells by adopting Tongxinluo, and culturing the treated mesenchymal stem cells to collect the exosome secreted by the mesenchymal stem cells. According to the mesenchymal stem cell source exosome, the mesenchymal stem cell source exosome capable of promoting angiogenesis and/or myocardial repair can be prepared.

Description

technical field [0001] The present invention relates to a mesenchymal stem cell-derived exosome based on drug pretreatment and its preparation method, in particular to a high-efficiency mesenchymal stem cell-derived exosome based on drug Tongxinluo pretreatment and its preparation method. Background technique [0002] WHO statistics show that in 2016, cardiovascular disease was the leading cause of death in the world (about 17.6 million people, 32.2%). Although the current medical level has been greatly improved, acute myocardial infarction (acute myocardial infarction, AMI) is still the leading cause of death worldwide. One of the main causes of disability (GBD 2016 Mortality Collaborators. Global, regional, and national under-5 mortality, adult mortality, age-specific mortality, and life expectancy, 1970-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet. 2017 Sep 16; 390(10100):1084-1150; Roth, G.A., et al., Global, Regional, and National Bur...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/0775A61K35/28A61P9/10
CPCC12N5/0662C12N5/0663C12N5/0667A61K35/28A61P9/10C12N2509/00C12N2500/76C12N2500/82
Inventor 杨跃进熊语嫣汤瑞杰
Owner FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap