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Use of a map3k10 gene fragment and primers in the preparation of intracranial aneurysm detection kits

A technology for intracranial aneurysm and gene promoter region, which is applied in DNA/RNA fragments, biochemical equipment and methods, and the determination/inspection of microorganisms. Effect

Active Publication Date: 2022-05-24
NINGBO FIRST HOSPITAL
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Problems solved by technology

At present, there are no published research reports on the relationship between MAP3K10 gene methylation and intracranial aneurysms at home and abroad

Method used

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  • Use of a map3k10 gene fragment and primers in the preparation of intracranial aneurysm detection kits
  • Use of a map3k10 gene fragment and primers in the preparation of intracranial aneurysm detection kits
  • Use of a map3k10 gene fragment and primers in the preparation of intracranial aneurysm detection kits

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Embodiment Construction

[0020] The present invention will be further described in detail below with reference to the embodiments of the accompanying drawings.

[0021] Use of a set of MAP3K10 methylation amplification primers in preparing an intracranial aneurysm detection kit, the detection kit includes a pair of MAP3K10 gene methylation specific amplification primers and a methylation specific sequencing Primers:

[0022] Nucleotide sequences of methylation-specific upstream primers:

[0023] 5'-ATTTGGAGTTGGAGAGTTTTAAG-3';

[0024] Nucleotide sequences of methylation-specific downstream primers:

[0025] 5'-Biotin-CTAAATACAAAACCCCTCACTCACCAAAAC-3';

[0026] Nucleotide sequences of methylation-specific sequencing primers:

[0027] 5'-GAGAGTTTTAAGAAGGATTT-3'.

[0028] An application of a detection kit for auxiliary diagnosis of intracranial aneurysm, the detection kit can be used for auxiliary diagnosis of intracranial aneurysm, prognosis of curative effect or screening of drugs.

[0029] 1. Co...

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Abstract

The invention discloses the development and application of a MAP3K10 gene fragment clinical diagnosis detection kit for intracranial aneurysms. The detection kit includes a pair of MAP3K10 gene methylation-specific amplification primers and a methyl Methylation specific sequencing primer: wherein the upstream primer has the nucleotide sequence shown in SEQ ID NO.1, the downstream primer has the nucleotide sequence shown in SEQ ID NO.2, and the methylation specific sequencing primer is as SEQ ID NO.2 As shown in ID NO.3, the advantage is that the diagnostic kit can conveniently and quickly detect intracranial aneurysms at the molecular level, with high detection efficiency and strong pertinence. At the same time, the MAP3K10 gene methylation-targeted Drugs are expected to become a new method for auxiliary diagnosis, detection and screening of intracranial aneurysms.

Description

technical field [0001] The invention relates to the technical field of auxiliary diagnosis of intracranial aneurysm, in particular to the development of a MAP3K10 gene fragment in the field of clinical diagnosis and detection kits for intracranial aneurysm and the application of curative effect monitoring and prognosis judgment. Background technique [0002] Intracranial aneurysms (IA) is a cerebrovascular disease with a high mortality rate, which mainly refers to the abnormal expansion of the lumen of the intracranial artery due to the fragile and abnormal expansion of the intracranial artery to form a prominent tumor. Most IAs are asymptomatic before rupture, and once they rupture, the mortality rate is as high as 40%, and half of them die within 48 hours. Even if the patient survives, 1 / 3 of the patients can have rebleeding, and the mortality rate of those who have rebleeding is as high as 70-80%. The age of onset of IA is generally between 40 and 50 years old, and the o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/154C12Q2600/136
Inventor 黄毅孙杰高翔聂晟
Owner NINGBO FIRST HOSPITAL
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