Method for observing egg laying amount of Frankliniella occidentalis

A Western flower thrips and quantity technology, which is applied in the field of observing the egg production of Western flower thrips, can solve problems such as violations, cannot guarantee egg survival, cannot be used to investigate egg hatching rate, etc., and achieves survival guarantee and accurate statistics. Effect

Pending Publication Date: 2021-09-07
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is contrary to the oviposition habit of western flower thrips, which will result in fewer eggs laid by western flower thrips, and the survival of eggs cannot be guaranteed, so it cannot be used to investigate the hatching rate of eggs, and the results obtained cannot represent western flower thrips. True Fecundity of Horses in Natural Conditions

Method used

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  • Method for observing egg laying amount of Frankliniella occidentalis
  • Method for observing egg laying amount of Frankliniella occidentalis
  • Method for observing egg laying amount of Frankliniella occidentalis

Examples

Experimental program
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Embodiment 1

[0025] Weigh the agar powder in a beaker with a concentration of 3%, add water, heat until viscous gel, pour it into a petri dish made of polystyrene with a size of 85*15mm, cool and condense to a solid state, and the agar solid is used for Moisturize the leaves of the plants you add later.

[0026] Take the cabbage leaves and wash them, put the petri dish upside down on the leaves as a cutting tool, rotate and cut the cabbage leaves to form a circular leaf disc with the same size as the inner bottom of the petri dish, place the leaf disc on the solid agar, press gently to make The two are tightly fitted together to obtain an agar leaf dish, which is reserved for use, such as figure 1 shown. Parafilm is Parafilm PM996.

[0027] The female thrips occidentalis adults (aged 0-1 days) were sucked into the prepared agar leaf dish petri dish with sucker pipette respectively, and after the petri dish was covered, the gap was sealed with a parafilm.

[0028] The culture dish was pl...

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Abstract

The invention belongs to the field of insect research, and particularly relates to a method for observing the egg laying amount of Frankliniella occidentalis, which comprises the following steps: firstly, manufacturing an agar leaf butterfly culture dish, then putting female adult Frankliniella occidentalis on the leaf butterfly culture dish, sealing the leaf butterfly culture dish with a cover, putting the leaf butterfly culture dish in an incubator, transferring the female adult Frankliniella occidentalis into a new leaf butterfly culture dish for two days, repeating for seven times, and then observing the number of laid eggs and the number of newly hatched nymphs in the culture dish after the female Frankliniella occidentalis is removed through a microscope. By means of the method, the living environment of female Frankliniella occidentalis under the natural condition can be restored, the egg laying number is accurate and can be directly observed through the microscope, the awkward situation that at present, counting of the egg laying amount of the Frankliniella occidentalis can only be replaced by the number of hatched one-year nymphs is solved, one-step biological observation can be carried out, and a method foundation is laid for biological research of thrip insects.

Description

technical field [0001] The application belongs to the field of insect research, and in particular relates to a method for observing the amount of eggs laid by Thrips occidentalis. Background technique [0002] Western flower thrips Frankliniella occidentalis (Pergande) belongs to Thysanoptera (Thysanoptera), Thripidae (Thripidae), is a major agricultural pest worldwide. The insect has a short developmental generation cycle, strong fecundity, miscellaneous feeding habits, and the ability to reproduce parthenogenetically, making its control extremely difficult. In addition to eating the stems, leaves, flowers and fruits of plants and causing the plants to wither, Western flower thrips can also transmit a variety of plant viruses including tomato spotted wilt virus (TSWV). Western flower thrips has a wide range of hosts and miscellaneous feeding habits. Its host plants include cucumbers, potatoes, eggplants, peppers, tomatoes, beans, strawberries, grapes, lettuce, orchids, chr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 吴青君华登科袁江江陶敏张镇
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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