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Agrobacterium capable of efficiently degrading ethyl carbamate and application of agrobacterium

A technology of urethane and Agrobacterium, applied in the field of microorganisms, can solve the problems of poor degradation of EC

Active Publication Date: 2021-09-03
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Aiming at the problem that the existing EC degrading bacteria agent has poor effect on EC degradation, the purpose of the present invention is to provide a strain of Agrobacterium DL-DNH01 and a degrading agent prepared by the Agrobacterium

Method used

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  • Agrobacterium capable of efficiently degrading ethyl carbamate and application of agrobacterium
  • Agrobacterium capable of efficiently degrading ethyl carbamate and application of agrobacterium
  • Agrobacterium capable of efficiently degrading ethyl carbamate and application of agrobacterium

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Experimental program
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Embodiment

[0042] Isolation and purification of strain DL-DNH01:

[0043] (1) Sample: collected from the soil of Liulin Town, Baoji, Shaanxi.

[0044] (2) The separation and screening method adopts the enrichment culture method, as follows:

[0045] Take 5 g of soil samples and add them to 50 mL of LB medium, and add EC mother liquor at the same time, so that the final mass concentration of EC is 1 g / L, and shake culture at 30 ° C and 200 rpm to enrich EC degrading bacteria. After culturing for 2 days, they were transferred to the second batch of LB medium with an EC concentration of 2 g / L at a ratio of 1:10. After 2 days of culture under the same conditions, they were transferred to the third batch of LB medium with an EC concentration of 4 g / L at a ratio of 1:10, and continued to culture for 2 days. After 2 days of culture under the same conditions, they were transferred to the fourth batch of LB medium with an EC concentration of 8 g / L at a ratio of 1:10, and continued to culture fo...

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Abstract

The invention provides agrobacterium DL-DNH01 capable of efficiently degrading ethyl carbamate. The preservation number of the agrobacterium DL-DNH01 is CGMCCNo.21308. The agrobacterium DL-DNH01 is subjected to activation, fermentation and microcapsule preparation to obtain a degradation preparation. The screened DL-DNH01 can be used for removing EC in fermented food, the degradation rate of the ethyl carbamate reaches 95%, the problem that toxic and harmful metabolites in the fermented food exceed the standard and the safety problem are solved, high-quality development of the safe fermented food is supported, and the quality and safety of alcoholic beverages and the fermented food are effectively improved. The invention further provides the degradation preparation produced and prepared by using the bacterial strain DL-DNH01. The degradation preparation has the advantages of being low in production cost, convenient to use, obvious in removal effect, easy to remove from a fermented product system and the like, and is suitable for removing EC in alcoholic beverages and fermented food.

Description

technical field [0001] The present invention relates to the technical field of microbes, and more specifically, relates to a soil-isolated Agrobacterium with EC degradation activity and its application, which is named Agrobacterium DL-DNH01 and classified as Agrobacterium tumefaciens. Background technique [0002] Ethyl carbamate (EC) is a metabolite with genotoxicity and strong carcinogenicity, widely present in alcoholic beverages and fermented foods (such as fermented bean curd, soy sauce, cheese, vinegar, pickles, etc.) Food safety and human health have serious impacts and hazards. [0003] EC in fermented foods is mainly formed by the reaction of carbamoyl-containing precursors such as urea, carbamoyl phosphate, citrulline, and diethyl pyrocarbonate with ethanol. EC in fermented foods can be removed by process optimization, metabolic engineering, microbial degradation and enzymatic methods. [0004] Among them, microbial degradation and enzymatic removal use EC degrad...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10C12N11/14A23L5/20C12R1/01
CPCC12N1/20C12N11/10C12N11/14A23L5/28A23L5/27Y02W30/62
Inventor 张素芳董乃慧林心萍梁会朋纪超凡薛思宇熊可欣
Owner DALIAN POLYTECHNIC UNIVERSITY
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