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Backcross method of improving sex-linked balanced lethal line of bombyx mori

A balanced lethal and chain-linking technology, applied in animal husbandry and other fields, can solve the problems of poor economic traits and no direct use value of introduced species, and achieve the effect of small-scale breeding and improvement, rapid improvement, and fast economic traits

Inactive Publication Date: 2004-01-07
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

Therefore, the economic traits of the introduced species are very poor, far inferior to the current silkworm species in our country, and have no direct use value in our country. The introduced species must be improved by transgenic

Method used

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  • Backcross method of improving sex-linked balanced lethal line of bombyx mori
  • Backcross method of improving sex-linked balanced lethal line of bombyx mori
  • Backcross method of improving sex-linked balanced lethal line of bombyx mori

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Embodiment Construction

[0013] 1. Balance the sex control gene structure of the lethal line

[0014] figure 1 It is the structure and principle of sex continuous balance lethal sex control genes. from figure 1 It can be seen that the two sex chromatids Z of the male of this system each carry a recessive homozygous embryonic lethal gene with a mutation, namely l 1 and l 2 . The exchange rate of the two genes is 0.8%; there is a recessive homozygous second albumen gene w on the 10th autosome 2 / w 2 , so the male gene structure is ,w 2 / w 2 (according to the standard genotype should be written as ,w 2 / w 2 , for the convenience of writing, the normal gene is deleted without causing confusion. ) Simultaneous translocation of the sex-linked lethal gene l on the W sex chromosome of the female 1 and the second albumen w 2 allelic normal gene fragment and , the Z chromosome also carries the lethal mutation gene l 1 ; On the 10th normal chromosome, there is also a homozygous 2nd albumen ...

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Abstract

The present invention discloses a method for improving Bombyx mori sec-linked balanced lethal line by using backcross process. Which is characterized by that it adopts three processes of crossing, selfing and backcross of balanced lethal line and conventional quality variety to form haematodes circulation, and utilizes said haematodes circulation to progressively substitute the gene of quality variety into the Bombyx mori sec-linked balanced lethal line, at the same time adopts marker gene selection measure to raise its economic trait on the premise of that the sex-controlling gene of balanced lethal line is not lost to reach the economic trait level of quality variety. Said invention is simple in operation method, and easy to popularize.

Description

technical field [0001] The invention relates to a method for backcrossing to improve the sex-linked equilibrium lethal line of silkworm. Background technique [0002] Compared with female silkworms, male silkworms have the obvious advantages of eating less mulberry, lower cost of raising silkworms, high silk yield and excellent silk quality. The special breeding of male silkworms can increase the economic benefit by more than 10% compared with the current mixed breeding of males and females. Therefore, countries such as China, Japan, and Russia have studied silkworm sex control technology from different angles, but have not obtained satisfactory male silkworm varieties for half a century, so the technology of exclusively raising male silkworms has not been applied in production practice. After decades of hard work, the Institute of Developmental Biology of the Russian Academy of Sciences has constructed a sex-linked balance lethal line of silkworms...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/04
Inventor 何克荣夏建国祝新荣黄健辉
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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