Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker co-separated from watermelon peel covering line gene ClGS and application of molecular marker

A molecular labeling and co-separation technology, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, food processing, etc. handy effect

Active Publication Date: 2021-08-31
HENAN AGRICULTURAL UNIVERSITY
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the related genes controlling the formation of watermelon peel pattern, and its regulatory network is still blank.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker co-separated from watermelon peel covering line gene ClGS and application of molecular marker
  • Molecular marker co-separated from watermelon peel covering line gene ClGS and application of molecular marker
  • Molecular marker co-separated from watermelon peel covering line gene ClGS and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Acquisition of Molecular Markers Closely Linked to Watermelon Plant Dark Green Ribbon Gene

[0031] biomaterials:

[0032] Watermelon material WM204, its peel is light green, covered with net pattern;

[0033] The watermelon material WT2 is a high-generation inbred line selected by the inventor. The fruit is light green peel and covered with dark green band-like patterns on the fruit surface. This material can be obtained through commercial channels or obtained from Henan Agricultural University. Provided by the Genetics and Breeding Research Group (it needs to be explained that the use of this material as a research basis is only for the convenience of obtaining experimental materials, and it should not be understood that the realization of the relevant technical solutions of this application must rely on this experimental material);

[0034]The above-mentioned watermelon material WT2 is consistent with the normal watermelon material WT2 with tendrils used i...

Embodiment 2

[0071] Example 2 Development of Indel1 marker

[0072] On the basis of Example 1, the inventors further performed a bioinformatics analysis on the candidate segment of the watermelon dark green band gene ClGS, and developed an Indel1 marker co-segregated with it. The specific process is as follows.

[0073] (1) Parental resequencing sequence analysis

[0074] On the basis of the fine mapping in Example 1, the inventors further conducted a bioinformatics analysis on the candidate segment of the watermelon dark green banded pattern gene ClGS. We took the genome sequence between dCAPS3 and dCAPS8 as the reference sequence, and according to the annotation of the reference genome, there were 11 genes in the candidate interval. Then, we mapped the original read length on the reference sequence, and analyzed it through software such as hisat2 and samtools. We found a total of 64 SNPs and 3 Indel sites in the CDS.

[0075] (2) Electronic BSA

[0076] On the basis of step (1), in o...

Embodiment 3

[0088] Example 3 Indel1 molecular marker function verification

[0089] On the basis of the above-mentioned embodiments, based on the collected watermelon germplasm materials from all over the world, the inventor randomly selects 25 parts of materials as an example (and the watermelon germplasm materials are all collected during the inventor's work, relevant Germplasm materials are also preserved in some domestic and foreign professional germplasm banks, and the following work is only for experimental verification, and the relevant germplasm materials are not directly related to the protection subject of this application, so these germplasm materials are no longer provided information), the Indel1 molecular marker function was further verified, and the specific process is briefly introduced as follows.

[0090] (1) Extract watermelon genomic DNA

[0091] Genomic DNA was extracted from unexpanded young leaves after seedlings (collected from the parental dark green banded-patte...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a pair of molecular markers co-separated from a watermelon dark green banded peel coating line gene ClGS and application, and belongs to the technical field of biology. The molecular marker can be directly used for molecular marker-assisted breeding of watermelon peel coating line materials, breeding selection efficiency is improved, the breeding process is accelerated, in addition, peel coating line characters of watermelon plants can be accurately and rapidly identified in the budding stage or the seedling stage by means of the molecular marker, and the molecular marker has the advantages of convenient detection and stable amplification product.

Description

technical field [0001] The invention belongs to the technical field of molecular breeding, and in particular relates to a molecular marker co-separated with the watermelon peel gene ClGS and its application. Background technique [0002] In recent years, with the improvement of people's living standards, the demand for high-quality horticultural products with rich nutrition, unique taste and diverse appearance is also increasing. As an important horticultural crop, watermelon has a very important position in the world's horticultural crops. As the world's largest producer and consumer of watermelon, China plays an important role in rural revitalization and optimization of agricultural industrial structure. Watermelon fruit appearance is an important trait of fruit appearance quality, which directly affects consumers' desire to buy to a certain extent. Therefore, in the breeding process, fruit appearance traits, as an important economic trait, have always been an important ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156Y02P60/87
Inventor 杨路明王登科杨森豆峻岭朱华玉刘东明
Owner HENAN AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com