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Molecular marker AhyRscc closely linked with deep red seed coats of peanuts and application of molecular marker AhyRscc

A molecular marker, deep red technology, applied in the field of agricultural biology, can solve the problems of peanut variety restrictions, achieve reliable results, improve breeding efficiency, and simple technical requirements

Active Publication Date: 2021-08-27
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The seed coat of peanuts is also developed from the integument. Compared with other traits, the color of the seed coat can only appear at a different generation. This characteristic has greatly restricted the cultivation of peanut varieties with deep red seed coats by traditional breeding methods.
However, there are no reports on the genes and molecular markers related to peanut crimson seed coat

Method used

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  • Molecular marker AhyRscc closely linked with deep red seed coats of peanuts and application of molecular marker AhyRscc
  • Molecular marker AhyRscc closely linked with deep red seed coats of peanuts and application of molecular marker AhyRscc
  • Molecular marker AhyRscc closely linked with deep red seed coats of peanuts and application of molecular marker AhyRscc

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1 Controlling the positioning of the peanut crimson seed coat color gene and the design of the molecular marker AhyRscc closely linked with the peanut crimson seed coat

[0036] In order to locate and control the color gene of peanut crimson seed coat, the inventors used the pink seed coat peanut variety (female parent) Yuanza 9102 to cross with the deep red seed coat peanut variety (male parent) Zhonghua 12 to construct a segregation population, genetic Analysis reveals that seed coat color in peanuts is controlled by a single gene.

[0037] by F 2 and F 3 For the phenotype analysis of the generation, 33 extreme red and 33 extreme pink materials were selected to construct the deep red and pink extreme pools respectively, and the whole genome resequencing was carried out together with the parents Zhonghua 12 and Yuanza 9102, and the parents and extreme The sequencing volumes of the pools are 30Gb and 90Gb respectively ( figure 1A). The resequencing data were...

Embodiment 2

[0039] Example 2 Using the molecular marker AhyRscc to rapidly breed a new high-yield crimson peanut variety

[0040] By using the molecular marker AhyRscc for selection combined with backcross breeding, common peanuts (recurrent parents) can be improved into new varieties of peanuts with dark red seed coats in about 3 years, and more than 97% of the genetic background of the recurrent parents can be retained. On the premise of retaining most of the excellent traits of the original peanut varieties, the directional improvement of the color of the seed coat from pink to deep red is realized.

[0041] The common peanut variety Fenghua No. 1 was used as the recurrent parent, and the deep red peanut variety Zhonghua No. 12 was used as the donor male parent. The specific steps for improving the color of the peanut dark red seed coat include:

[0042] (1) hybridization

[0043] HF001 was used as the female parent (reincarnation parent) and the dark red peanut variety Zhonghua No. 1...

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Abstract

The invention discloses a molecular marker AhyRscc closely linked with deep red seed coats of peanuts and application of the molecular marker AhyRscc, which belong to the technical field of agricultural biology. The specific primer pair of the molecular marker AhyRscc comprises primers with nucleotide sequences as shown in SEQ ID No.1 and SEQ ID No.2; the molecular marker can effectively identify the color of the dark red seed coat of the peanut, on one hand, fine positioning, separation and cloning of genes are facilitated, and on the other hand, the molecular marker has important application value for molecular breeding and quality improvement of the peanut.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a molecular marker AhyRscc closely linked with peanut crimson seed coat and its application. Background technique [0002] Peanut is an important oil crop and economic crop in my country, with an annual planting area of ​​about 70 million mu and an output of about 17 million tons. Peanuts are rich in fatty acids, proteins, vitamin E, calcium, iron, zinc, selenium and other trace elements, and are also rich in functional active substances such as resveratrol, which have important health care functions. They are called "longevity fruit" among the people. Peanut seed coats, especially dark red ones, are rich in natural pigments, especially anthocyanins, which have various biological effects such as inhibiting free radicals and anti-oxidation (Zhang Yufang, 2003). In addition, peanut seed coat, also known as peanut red coat, is a traditional Chinese medicine that can stop b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 赵传志王兴军袁美张鲲侯蕾厉广辉田锐铮夏晗李膨呈赵术珍李长生李爱芹潘教文
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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