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A kind of oral insulin chitosan nanoparticle solution and preparation method thereof

A chitosan nanoparticle and insulin technology is applied in pharmaceutical formulations, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc. It can solve the problems of low biological activity and easy degradation of insulin, and achieve biological High compatibility, promotion of encapsulation, and high stability

Active Publication Date: 2022-05-17
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of oral insulin chitosan nanoparticle solution and preparation method thereof, in order to solve the technical problem that insulin is easily degraded by oral administration of insulin, and biological activity is low

Method used

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  • A kind of oral insulin chitosan nanoparticle solution and preparation method thereof
  • A kind of oral insulin chitosan nanoparticle solution and preparation method thereof
  • A kind of oral insulin chitosan nanoparticle solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the preparation of chitosan nanoparticle CNP

[0045] Fully dissolve chitosan (CS) with low viscosity (5-20mPa·s) in 1% acetic acid solution, the concentration is 1.5mg / mL; then the pH value of the solution is adjusted to 5.5; Sodium tripolyphosphate (TPP ) was dissolved in deionized water at a concentration of 0.5 mg / mL, and the pH was adjusted to 7.6; the TPP solution (0.5 mg / mL) was added dropwise to the chitosan solution (1.5 mg / mL), and then stirred at 25 ° C for 60 Minutes, the stirring speed was 600rpm / min, and after 10 minutes of reaction, high-speed centrifugation obtained chitosan nanoparticles CNP, wherein the mass ratio of CS:TPP was 5:1.

Embodiment 2

[0046] Embodiment 2: the preparation of HCP and INS-HCP

Embodiment 2-1-1

[0047] Example 2-1-1: Preparation of HCP-1-1

[0048] Sodium hyaluronate was dissolved in distilled water, and the pH was adjusted to 5.5 with 0.5M acetic acid buffer to prepare a hyaluronic acid solution. The concentration of the sodium hyaluronate solution was 0.1 mg / mL; CNP (1.5mg / mL, pH5.5) solution prepared by the method, and then the mixed solution was stirred at room temperature for 30 minutes at a stirring speed of 600rpm / min to obtain HA-CS / TPP (HCP ), wherein the mass ratio of HA:CNP is 8:1.

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Abstract

The invention discloses an oral insulin chitosan nanoparticle solution and a preparation method thereof. The nanoparticle is prepared by ion cross-linking reaction of chitosan and sodium tripolyphosphate (TPP) in the solution, and hyaluronic acid is adsorbed by charge. The effect is wrapped on the surface of chitosan nanoparticles. As the carrier of insulin, the nanoparticles protect insulin from passing through the stomach after oral administration, avoiding the damage of gastric acid and protease to insulin. After reaching the small intestine, the nanoparticles are absorbed by the intestinal cells and enter the blood circulation. system to lower and control blood sugar levels. The nanoparticle solution disclosed by the invention has a simple preparation process, uses natural biological materials, avoids toxic and harmful chemical reagents, and has high stability during storage at room temperature, high drug loading efficiency of insulin, good slow-release effect in the stomach, and chemical The structure and biological activity do not change, and it has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to an oral insulin chitosan nanoparticle solution and a preparation method thereof. Background technique [0002] Nationally and worldwide, with the aging population and changes in living habits, the number of people with diabetes is increasing year by year, and the health management of people with diabetes is an important personal and social issue. Although some drugs, such as Glucophage and some Chinese herbal preparations, have a good effect on controlling blood sugar, insulin is still the most effective and reliable drug for controlling blood sugar in patients with advanced and severe diabetes. Insulin has a molecular weight of 5.8kDa and is composed of 2 peptide chains consisting of 51 amino acid residues. It is the main hormone that lowers blood sugar in the human body. [0003] Insulin is currently administered by subcutaneous injection in the abdomen....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K38/28A61K47/36A61P3/10
CPCA61K38/28A61K9/5161A61P3/10
Inventor 李井雷杨柳吴海山
Owner HEFEI UNIV OF TECH
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