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Single emulsifier based on DNA triangular origami technology and preparation method and application of double emulsion

A technology of double emulsion and emulsifier, applied in cosmetic preparations, preparations for skin care, medical preparations containing active ingredients, etc., to achieve the effect of good stability and simple operation

Active Publication Date: 2021-08-27
浙江百强乳业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, arbutin is a hydrophilic substance and coumaric acid is a hydrophobic substance, and there are challenges in its application at the same time

Method used

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  • Single emulsifier based on DNA triangular origami technology and preparation method and application of double emulsion
  • Single emulsifier based on DNA triangular origami technology and preparation method and application of double emulsion
  • Single emulsifier based on DNA triangular origami technology and preparation method and application of double emulsion

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0043] A kind of preparation method based on the single emulsifier stable double emulsion of DNA triangular origami technology, concrete steps are as follows:

[0044] (1) see figure 1 , each block unit of DNA triangular origami was designed in caDNAno software, and DNA staple chains were obtained at the same time, as shown in Table 1:

[0045] In sequence SEQ ID NO.2, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.10, SEQ ID NO.13, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.24, SEQ ID NO.31, SEQ ID NO.36, SEQ ID NO.43, SEQ ID NO.47, SEQ ID NO.54, SEQ ID NO.57, SEQ ID NO.62, SEQ ID NO. 63, SEQ ID NO.65, SEQ ID NO.66, SEQ ID NO.67, SEQ ID NO.68, SEQ ID NO.72, SEQ ID NO.77, SEQ ID NO.78, SEQ ID NO.82, SEQ ID NO.82, SEQ ID NO. ID NO.83, SEQ ID NO.87, SEQ ID NO.97, SEQ ID NO.99, SEQ ID NO.105, SEQ ID NO.111, SEQ ID NO.112, SEQ ID NO.116, SEQ ID NO. 119, SEQ ID NO.126, SEQ ID NO.129, SEQ ID NO.135, SEQ ID NO.136, SEQ ID NO.139, SEQ ID NO.141, SEQ ID NO.142, SEQ ID NO.145, SEQ ID N...

Embodiment 1

[0081]Both the inner and outer water phases of the W / O / W emulsion of Example 1 contained 10 μM of the DNA triangular origami of Example 1, and the inner and outer water phases of the W / O / W emulsion of Comparative Example 1 contained 10 μM of the DNA triangular origami of Comparative Example 1, Both the inner and outer water phases of the W / O / W emulsion of Comparative Example 2 contained 5 μM of the DNA triangular origami of Comparative Example 2, the oil phase of the W / O / W emulsion of Comparative Example 3 contained 10 μM of PGPR, and the outer aqueous phase contained 10 μM of Tween 80 . To study in vitro release, 1 mL of the prepared W / O / W emulsion was placed in PBS solution at physiological pH=7.4. The mixture was shaken at 200 rpm and then centrifuged at different time intervals (4, 8, 12, 16, 20 and 24 hours). The HPLC method was used to evaluate the in vitro release profile.

[0082] In vivo whitening experiment in zebrafish

[0083] Both the inner and outer water phas...

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Abstract

The invention discloses a single emulsifier based on a DNA triangular origami technology and a preparation method and application of a double emulsion. The preparation method comprises the following steps: designing and synthesizing a staple chain of DNA triangular origami; mixing a DNA skeleton chain and the staple chain to synthesize DNA triangular origami; purifying and enriching the triangular origami; and preparing the double emulsion. The process is simple, the obtained DNA triangular origami can serve as a single emulsifier of the double emulsion, the prepared emulsion can deliver hydrophilic arbutin and hydrophobic coumaric acid at the same time, and a center nano hole of the DNA triangular origami can serve as a release nano channel of the arbutin and the coumaric acid. Zero-order release is realized under the driving of intermolecular acting force of arbutin, cumaric acid and DNA triangle origami and aperture confinement effect. The synthesis steps are few, the operation is simple, and the application in actual production is facilitated.

Description

technical field [0001] The invention relates to the technical field of DNA nanostructure preparation, in particular to a single emulsifier-stabilized double emulsion based on DNA triangular origami technology and the application of the double emulsion as a phenolic substance delivery system. Background technique [0002] The Watson-Crick principle of complementary base pairing makes the structural characteristics of DNA predictable. In 1982, Seeman proposed the use of DNA as a building material to assemble geometrically shaped objects with nanoscale features. This revolutionary idea laid the foundation for an emerging field of research, today known as "structural DNA nanotechnology." Taking advantage of the self-recognition properties of DNA, rigid branched DNA patterns were developed based on complementary Watson-Crick base pairs between fragments of a given oligonucleotide set. In 2006, Rothemund introduced the scaffold-based DNA origami technology and achieved a remarka...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/63A61K8/60A61K8/06A61K8/365A61Q19/02
CPCA61K8/602A61K8/365A61K8/63A61K8/606A61K8/066A61Q19/02A61K2800/82A61K2800/56A61K2800/592C12N15/11C12N2310/3515B82Y5/00A61K8/06C12N15/111
Inventor 罗自生黄皓徐艳群林星宇李莉
Owner 浙江百强乳业有限公司
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