Method for rapidly detecting ethyl carbamate in white spirit based on ultra-high performance liquid chromatography combined with high resolution mass spectrometry

Abstract

The invention discloses a method for rapidly detecting ethyl carbamate in baijiu based on ultra-high performance liquid chromatography combined with high resolution mass spectrometry, and belongs to the technical field of detection and screening of risky substances in fermented food. The method includes the following steps: performing concentration and redissolution pretreatment on a white spirit sample, and extracting and enriching a target to-be-detected substance in the white spirit sample to obtain a target to-be-detected substance sample; detecting the obtained target to-be-detected object sample based on an ultra-high performance liquid chromatography-high resolution mass spectrometry technology to obtain a primary mass spectrum and a secondary mass spectrum of the target to-be-detected object sample; and extracting target object information through software based on the obtained primary and secondary mass spectrograms, establishing a standard spectrogram based on an ethyl carbamate standard substance, comparing the established standard spectrogram with the obtained extracted target object information, and carrying out qualitative and quantitative determination on ethyl carbamate in the white spirit sample. Thus, the high-sensitivity and high-accuracy systematic detection method for the food safety risk ethyl carbamate in the white spirit is established.

Description

technical field [0001] The invention belongs to the technical field of detection and screening of risk substances in fermented food, and in particular relates to a method for rapidly detecting ethyl carbamate in liquor based on ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry. Background technique [0002] Baijiu is a traditional distilled liquor in my country and one of the six major distilled liquors in the world. With its unique craftsmanship and style, it is unique among distilled liquors in the world and is deeply loved by the majority of drinkers. At present, our country pays more and more attention to food safety, and the requirements for liquor food safety are also constantly improving. Ethyl carbamate (EC), a compound with potential carcinogenic effects, is widely present in traditional fermented foods. During the brewing process of liquor, nitrogen-containing compounds in some raw and auxiliary materials will generate eth...

AI Technical Summary

Problems solved by technology

GC-MS is a conventional technique for the detection of EC in fermented foods, but the sample pretreatment of this method is complicated, and the appropriateness of sample preparation is directly related to the results of detection and analysis, which limits the application range of this technique to a certain extent

Compared with GC-MS, pre-column derivatization HPLC-FID analysis of ethyl carbamate has higher sensitivity, accuracy and reproducibility. technological development

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