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Application of recombinant cytokine gene derived protein or fragment thereof

A technology of recombinant cells and derived proteins, which is applied in the field of antiviral research and can solve problems such as the lack of specific treatment methods for diseases

Pending Publication Date: 2021-08-24
GENOVA BIOTECH QINGDAO CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there is no specific treatment for the disease caused by the new coronavirus, and it can only be treated according to the symptoms displayed by the patient

Method used

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  • Application of recombinant cytokine gene derived protein or fragment thereof
  • Application of recombinant cytokine gene derived protein or fragment thereof
  • Application of recombinant cytokine gene derived protein or fragment thereof

Examples

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Effect test

Embodiment

[0055] In this example, the 96-well plate method was used to carry out the detection of Novaferon (Novaferon, provided by Jiehua Biotechnology (Qingdao) Co., Ltd.) in vitro to inhibit the new coronavirus, as follows:

[0056]Vero cells were selected as the cell strain, and the plate was monolayered with a cell concentration of 10,000 cells / well (100 μl) for 24 hours, and then COVID-19 virus was selected as the virus strain, and 100 PFU / well was inoculated in each well. After the cells were infected with the virus for 2 hours, different concentrations of Lefuneng drugs were added to each well (concentrations were 0.001ng / ml, 0.01ng / ml, 0.1ng / ml, 1ng / ml, 10ng / ml, 100ng / ml ) as the treatment group, and the wells without Lefuneng drug as the control group.

[0057] After treating the cells with different concentrations of drugs for 24 hours, 100 μl of the cell supernatant in each well was taken for nucleic acid extraction (automatic nucleic acid extraction instrument from Tianlong...

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Abstract

The invention provides an application of a recombinant cytokine gene-derived protein or a fragment thereof in inhibiting the activity of a coronaviridae virus. The recombinant cytokine gene-derived protein comprises an amino acid sequence SEQ ID NO: 1, or an amino acid sequence having at least 90% identity with the amino acid sequence SEQ ID NO: 1. In addition, the invention also provides application of the recombinant cytokine gene derived protein or the fragment thereof in preparation of drugs for preventing or treating diseases or symptoms related to coronaviridae viruses in subjects. The recombinant cytokine gene derived protein or fragment thereof according to the present invention has enhanced anti-coronaviridae virus activity compared to human interferon [alpha]2b (HuIFN-[alpha]2b).

Description

technical field [0001] The present invention belongs to the field of antiviral research, and in particular relates to the use of a recombinant cytokine gene-derived protein or a fragment thereof for inhibiting the activity of Coronaviridae viruses; Use in medicine for a disease or symptom associated with a Coronaviridae virus. Background technique [0002] Coronavirus is a large virus family, which belongs to the genus Coronavirus of the order Nidovirales, the family Coronaviridae, and the genus Coronavirus in terms of systematic classification. Viruses of the genus Coronavirus are RNA viruses with an envelope and a linear single-stranded positive strand genome. They are a large class of viruses that exist widely in nature. The diameter of coronavirus is about 80-120nm, the 5' end of the genome has a methylated cap structure, and the 3' end has a poly(A) tail. The total length of the genome is about 27-32kb, which is the largest genome among known RNA viruses Virus. Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/19A61P31/14C07K14/525
CPCA61K38/191A61P31/14C07K14/525A61K38/19A61P31/12A61K38/21C07K14/555A61K31/56
Inventor 吕秋军刘龙斌
Owner GENOVA BIOTECH QINGDAO CO LTD
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