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Nanometer material capable of stably controlling NO release amount as well as preparation method and application of nanometer material

A nanomaterial, stable control technology, applied in the fields of nanotechnology, nanomedicine, nanotechnology, etc., can solve the problems such as the inability to sterilize and promote wound healing, the inability to effectively promote wound healing, and the inability to effectively solve the problem of wound healing. Healing effect, pro-healing effect, effect of maintaining vascular homeostasis

Active Publication Date: 2021-08-24
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While improving the stability of GSNO, the system also has better degradation performance on the wound surface, but the system still has the problem of slow NO release and cannot effectively promote wound healing.
[0007] But at the same time, the rapid release of NO in a short period of time will also lead to the exhaustion of NO in advance, which cannot achieve long-term bactericidal and healing-promoting effects on wounds, and cannot effectively solve the problem of wound healing. Therefore, a material is needed to accelerate At the same time as NO is released, the amount of NO released can be stably controlled within an appropriate range, so that the effect of promoting wound healing can be optimized

Method used

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  • Nanometer material capable of stably controlling NO release amount as well as preparation method and application of nanometer material
  • Nanometer material capable of stably controlling NO release amount as well as preparation method and application of nanometer material
  • Nanometer material capable of stably controlling NO release amount as well as preparation method and application of nanometer material

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preparation example Construction

[0041] According to the examples disclosed in the present invention, the present invention exemplarily proposes a method for preparing the above-mentioned nanomaterials that can stably control the amount of NO released, specifically including:

[0042] Sodium tripolyphosphate (TPP) and AgNO 3 Mix the solutions in proportion to obtain the first solution, mix chitosan (CS) and GSNO in an acidic environment in proportion to obtain the second solution, and then add the first solution dropwise to the second solution under stirring. The reaction is combined to form the third solution. After the reaction is completed, the reducing agent is added dropwise to the third solution under the condition of ultrasonication of the third solution, and the stirring reaction is continued for a period of time, and then the product is centrifugally washed to obtain the nanomaterial CS / TPP-GSNO-NPAg.

[0043] In a specific embodiment, in the first solution, TPP and AgNO 3 The volume ratio of the ...

Embodiment 1

[0056] Preparation of GSNO

[0057] Measure 0.2mL of concentrated HCl (12M) and mix and dilute it with 6mL of pure water to obtain an HCl solution, then weigh 616mg of glutathione (GSH) and slowly add it to the HCl solution under the conditions of ice-water bath and 500rpm stirring , and weighed 140mg sodium nitrite (NaNO 2 ) was also slowly added to the HCl solution, and the entire reaction system was stirred at a speed of 500 rpm for 30 minutes under the condition of avoiding light and an ice bath. After the reaction, the product was precipitated and washed with a mixture of acetone:water=4:1, and freeze-dried for 24 hours to obtain the final product GSNO.

[0058] Preparation of CS / TPP-GSNO-NPAg

Embodiment 2

[0060] Weigh 100mg of sodium tripolyphosphate and dissolve it in 20mL of deionized water to prepare a 5mg / mL sodium tripolyphosphate (TPP) solution, mix the TPP solution with 1mg / mL of AgNO 3 The solution is mixed according to (10:1=v:v) to obtain TPP-AgNO 3 solution.

[0061] Weigh 34mg of chitosan and dissolve it in 20mL of 0.1M HCl solution and stir on a turntable at 600rpm for 24h to prepare 1.7mg / mL of chitosan (CS) solution. Take 2.7mL of CS solution and mix 200μL of Example 1 The obtained GSNO solution (10 mg / mL) was added into the CS solution under the condition of stirring at 350 rpm to obtain a CS-GSNO solution.

[0062] Under the condition of 350rpm, 0.33mL of TPP-AgNO 3 The solution was added dropwise into the CS-GSNO solution, and after 30 minutes of reaction, TPP-AgNO 3 -CS-GSNO solution, then weigh 2.4mg sodium biborate (NaBH 4 ) was dissolved in 1mL of ice water to prepare 2.4mg / mL of NaBH 4 solution, and 20 μL was added dropwise to TPP-AgNO under ultrason...

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Abstract

The invention provides a nanometer material capable of stably controlling NO release amount. The nano-material is spherical nano-particles formed by sodium tripolyphosphate and chitosan. The spherical nano-particles have a cavity structure, nano-silver is carried on the inner surface and the outer surface of the cavity, an NO donor is entrapped in the cavity, and the NO donor is GSNO. The invention further provides a preparation method and application of the nanometer material capable of stably controlling the NO release amount. According to the nanometer material capable of stably controlling the NO release amount, through the synergistic effect of the nano-silver, the chitosan and the sodium tripolyphosphate, the NO release amount is stably controlled within a proper range, and long-acting sterilization can be achieved, so that the problem of wound healing can be effectively solved, and meanwhile, the material is low in toxicity, free of drug resistance and free of toxic effect on a human body.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a nanomaterial capable of stably controlling NO release, a preparation method and an application thereof. Background technique [0002] Diabetes mellitus (DM) is a prevalent chronic disease worldwide characterized by persistently high blood glucose levels. Data suggest that approximately 19–34% of diabetic patients are expected to develop diabetic foot ulcers (DFU), a common but complex and costly complication of chronic, difficult-to-heal wounds. Chronic wounds are wounds that do not heal normally after entering a pathological inflammatory state. Different from acute wounds, the formation of chronic wounds comes from continuous external stimuli. The cause of diabetes will lead to foot wounds to further become chronic wounds, that is, diabetic foot ulcers. [0003] The current clinical treatment methods for diabetic foot ulcers include wound debridement, antibiotic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/06A61K33/38A61K9/51A61K47/02A61K47/36A61P17/02A61P31/04B82Y5/00B82Y40/00
CPCA61K38/063A61K33/38A61K9/5115A61K9/5161A61P17/02A61P31/04B82Y5/00B82Y40/00A61K2300/00Y02A50/30
Inventor 任浩杨政李学明王永禄孟政杰吴培瑶白云浩
Owner NANJING UNIV OF TECH
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