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Ratio sensing detection method for activity of CeO2 imitated organophosphorus hydrolase and imitated oxide enzyme

A detection method, oxidase technology, applied in the field of agrochemicals, can solve the problems of complicated sample pretreatment, dependence on staff, and limitation of practical application, and achieve low price of equipment, less time consumption, and strong adaptability to temperature and environment Effect

Active Publication Date: 2021-08-20
QINGDAO AGRI UNIV
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Problems solved by technology

At present, the analysis methods commonly used in laboratories have problems such as complicated sample pretreatment, high cost, dependence on well-trained staff, and time-consuming, which limit their practical application.

Method used

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  • Ratio sensing detection method for activity of CeO2 imitated organophosphorus hydrolase and imitated oxide enzyme

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Embodiment 1

[0024] The present embodiment is based on the ratio sensing detection method of CeO imitating organophosphate hydrolase and imitating oxidase activity, the construction of ratio sensing detection method, the specific construction method is:

[0025] Step 1: Incubate standard samples of paraoxon at different concentrations with cerium dioxide nanozyme, centrifuge and take out the supernatant to measure UV-visible absorption to obtain p-nitrophenol signal A400;

[0026] Step 2: The precipitate obtained after centrifugation in step 1 was dissolved in acetic acid buffer solution, and then the above solution was reacted with the prepared TMB to measure the UV-visible absorption, and the absorption peak A653 of oxidized TMB was obtained. Finally, the signal of p-nitrophenol was compared with the signal of oxidized TMB.

[0027] The preparation method of the ceria nanozyme of this example is as follows: dissolve cerium chloride in 75mL of ultrapure water, then add ammonia water and h...

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Abstract

A ratio sensing detection method based on the activity of a CeO2 imitated organic phosphorus hydrolase and the CeO2 imitated oxide enzyme is characterized by comprising the specific steps: 1, incubating paraoxon standard samples with different concentrations and cerium dioxide nano enzyme, centrifuging, taking out supernate, and detecting ultraviolet and visible absorption to obtain a p-nitrophenol signal A400; and 2, dissolving the precipitate obtained after centrifugation in the step 1 with an acetic acid buffer solution, and then taking the solution to react with the prepared TMB to measure ultraviolet and visible absorption so as to obtain the absorption peak A653 of the oxidation-state TMB. Finally, the signal of the p-nitrophenol is compared with the signal of the oxidation-state TMB. The sensing method is low in experiment cost, high in accuracy, high in selectivity and high in detection speed in the detection process, and therefore paraoxon can be rapidly and sensitively detected through the designed CeO2-based organophosphorus-imitated hydrolase and oxygenase-imitated activity ratio sensing detection method.

Description

technical field [0001] The invention relates to the technical field of agrochemicals, in particular to a ratio sensing detection method for the activities of CeO2 imitating organophosphate hydrolase and imitating oxidase. Background technique [0002] Excessive pesticide residues will cause harm to the health of the eaters. Long-term consumption of vegetables or other agricultural products with excessive pesticide residues will reduce immunity and even cause death from poisoning. Organophosphorus pesticides are neurotoxicants that cause neurological disorders. At present, the analysis methods commonly used in laboratories have problems such as complicated sample pretreatment, high cost, dependence on well-trained staff, and time-consuming, which limit their practical application. The biosensing method has the advantages of simple and fast, high accuracy, good portability, low cost-effectiveness, and easy on-site detection, which reduces the time and cost required for sample...

Claims

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Application Information

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IPC IPC(8): G01N21/33
CPCG01N21/33Y02A50/20
Inventor 盖盼盼李峰宋盼盼蒲黎
Owner QINGDAO AGRI UNIV
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