Liquid culture method for rapidly increasing biomass of didymodon tectorum protonemata

A technology of liquid culture and anti-dental moss, applied in biochemical equipment and methods, microorganisms, cell culture medium, etc., can solve problems such as low reproduction efficiency, and achieve the effect of improving application potential and increasing biomass

Pending Publication Date: 2021-08-17
INNER MONGOLIA UNIVERSITY
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as the main source of transgenic materials, the growth state and quantity of the protonema are the main factors limiting the transgenic efficienc

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Liquid culture method for rapidly increasing biomass of didymodon tectorum protonemata
  • Liquid culture method for rapidly increasing biomass of didymodon tectorum protonemata
  • Liquid culture method for rapidly increasing biomass of didymodon tectorum protonemata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Materials and Methods

[0022] 1.1 Research materials:

[0023] The material used in the present invention is P. brevifolia, which is preserved in a laboratory incubator after several generations of plant tissue culture.

[0024] 1.2 Research methods:

[0025] 1.2.1 Experimental design: Inoculate the 30-day-old P. brevifolia protonema material in the improved liquid knop medium to observe the growth state, and finally determine the optimal nutrient ion concentration for the protonema growth state.

[0026] 1.2.2 Protonema tissue culture rapid propagation technology:

[0027] The configuration of the improved liquid medium: the improved Knop basal medium is used as the background medium, and the formula of the improved liquid medium is: sucrose 8g / L, calcium nitrate Ca(NO 3 ) 2 4H 2 O2.5g / L, potassium nitrate KNO 3 0.625g / L, potassium dihydrogen phosphate KH 2 PO 4 0.625g / L, magnesium sulfate MgSO 4 7H2Q0.625g / L, zinc sulfate ZnSO 4 ·7H 2 O7mg / L, ferrous ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a liquid culture method for rapidly increasing the biomass of didymodon tectorum protonemata. The liquid culture method comprises the following steps: taking didymodon tectorum spores, carrying out plant tissue rapid propagation culture on a solid Knop culture medium, carrying outsterile culture ofdidymodon tectorum on a solid culture medium forsubculture at a temperature of 24 DEG C, under a condition of 24 hours of illumination and 24 hours of dark, culturing in an incubator with the light intensity of 3000 lx for 1 week, taking the material, mechanically grinding and crushing, inoculating the material into an improved liquid Knop culture bottle by a pipette, culturing in the incubator with the temperature of 24 DEG C, the illumination of 12 hours, the darkness of 12 hours and the light intensity of 3000 lx for one month, observing the growth state of protonemata by using a microscope during the period, and measuring and counting the biomass of protonemata. According to the invention, a large amount of didymodon tectorum protonemata can be obtained in a short time, and the application potential of didymodon tectorum protonemata as a transgenic material subsequently is greatly improved.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a liquid culture method for rapidly increasing the biomass of P. Background technique [0002] Liverwort is one of the oldest terrestrial plants. Its evolutionary position is behind algae and before ferns and seed plants. It belongs to the sister clade of single-origin system with vascular plants, and has an extremely important research status. There are about 20,000 species in the world, all over every corner of the earth except the ocean, and even grow on deserts, tundra and rocks. They are drought-resistant, cold-resistant, and highly adaptable. They are the pioneers and pioneers of nature. [0003] Didymodon tectorum is a plant of the genus Didymodon in the family Pottiaceae. The plant body is relatively thick, yellow-green or slightly yellow-brown, densely clustered, 1-1.5cm high. This species belongs to the East Asia-North America distribution species, the distributio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/04C12N13/00
CPCC12N5/04C12N5/0025C12N13/00
Inventor 赵东平王丹王丽红杨倩
Owner INNER MONGOLIA UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap