Preparation and application of animal model with broad brain region neuron tree sudden fertility disorder

Abstract

The invention relates to preparation and application of an animal model with broad brain region neuron tree sudden fertility disorder. Specifically, a preparation method of a non-human mammal model with the broad brain region neuron tree sudden fertility disorder is provided and comprises the following steps: exposing the non-human mammal to a hypoxia condition for 30-40 minutes so as to obtain an animal model with the broad brain region neuron tree sudden fertility disorder, wherein under the hypoxia condition, the oxygen concentration (volume ratio) is reduced from 10-20% to 1-5%. The animal model can be used for researching the broad brain region neuron tree sudden fertility disorder, and can be used for screening and testing specific drugs.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the preparation and application of an animal model of developmental disorder of neurons and dendrites in a wide range of brain regions. Background technique [0002] Hypoxic / ischemic (H / I) injury is one of the major causes of deficits in brain function at all ages and has been extensively studied in both clinical and experimental animal studies, including etiology, neuropathogenesis, and pharmacological interventions. A growing body of research suggests that H / I may have adverse effects on rodent brain development. [0003] Most current animal models of hypoxic brain injury include: [0004] 1. Arterial blockage + hypoxia method - cerebral ischemia and hypoxia model: Disadvantages: anesthesia has neuroprotective effect; surgery itself has trauma; infarct variation is large and unstable; model production is cumbersome and batches are small; success rate is low; production The cycle ...

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Problems solved by technology

[0004] 1. Arterial blockage + hypoxia method - cerebral ischemia and hypoxia model: Disadvantages: anesthesia has neuroprotective effect; surgery itself has trauma; infarct variation is large and unstable; model production is cumbersome and batches are small; success rate is low; production long cycle

Not suitable for simulation of neonatal hypoxic ischemic encephalopathy

[0006] 3. Tracheal clamping method: The disadvantages are: the influence of anesthetic drugs on the nerves; the trauma caused by the operation itself; high technical requirements, long cycle; Poor consistency with pathological changes

[0007] 4. Establishing an intrauterine hypoxia model: Disadvantages: data impact of anesthesia drugs; surgical trauma; high surgical technical requirements; large consumption of surgical consumables; long cycle, small batch size; large variance and poor uniformity

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