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Method for detecting amantadine and rimantadine in tomatoes

A detection method, the technology of amantadine, applied in the field of material detection, can solve the problems of no plant source food amantadine and rimantadine, large matrix interference, cumbersome operation, etc., to achieve improved detection accuracy, low cost, The effect of improving sensitivity

Pending Publication Date: 2021-08-10
AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

The literature reports are all methods for the determination of amantadine and rimantadine in food of animal origin; the determination methods mainly include gas chromatography, high performance liquid chromatography, capillary electrophoresis and liquid chromatography-mass spectrometry, and the above determination methods have low sensitivity
The pretreatment method mostly adopts SPE solid phase extraction column purification method, the above pretreatment method is cumbersome to operate and has a lot of matrix interference
At the same time, there are no reports on the detection methods of amantadine and rimantadine residues in plant-derived foods, especially tomatoes.

Method used

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  • Method for detecting amantadine and rimantadine in tomatoes
  • Method for detecting amantadine and rimantadine in tomatoes
  • Method for detecting amantadine and rimantadine in tomatoes

Examples

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Embodiment 1

[0093] Extraction: Weigh 25.00g tomato sample into a 100mL stoppered test tube, add 100ng / mL D 15 - Add 50 μL of amantadine standard working solution (methanol as the solvent), add 7 g of NaCl and 50 mL of formic acid acetonitrile solution with a volume concentration of 0.1% formic acid, and extract by vortexing at 5000 rpm for 3 min to obtain an extract.

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Abstract

The invention belongs to the technical field of substance detection, and particularly provides a method for detecting amantadine and rimantadine in tomatoes. According to the method, a PSA adsorbent, a C18 adsorbent and anhydrous magnesium sulfate are adopted as purification reagents, impurities which interfere with a target object in an extract solution can be adsorbed, the technical problem that matrix interference is serious is solved, and the detection accuracy is improved; and by adopting liquid chromatography-tandem mass spectrometry detection, the sensitivity of the detection method is greatly improved. The detection method provided by the invention is less in used solvent and low in cost. Furthermore, the accuracy, the precision and the sensitivity of the detection method are improved by further limiting liquid chromatography-tandem mass spectrometry detection parameters.

Description

technical field [0001] The invention relates to the technical field of substance detection, in particular to a method for detecting amantadine and rimantadine in tomato. Background technique [0002] Amantadine (AMT) is an amino derivative of saturated tricyclodecane, also known as amantadine, tricyclodecane, etc., and was synthesized in 1960. It was first used as an antiviral drug and was the first to be approved by the FDA. anti-influenza drugs. Rimantadine (RMT) is a drug obtained by replacing the amino group of amantadine with an ethylamine group. This drug was approved by the United States in 1993 as an antiviral drug for the prevention and treatment of influenza A virus. Most of the amantadine and rimantadine used in the breeding process are excreted with feces and urine, resulting in high levels of antibiotics (such as amantadine and rimantadine) in the feces. Farming waste in our country is becoming an important source of antibiotics in the environment and has attr...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/14G01N30/72
CPCG01N30/02G01N30/14G01N30/72G01N2030/027
Inventor 查玉兵王晓芳叶剑芝杨春亮曾绍东林玲
Owner AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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