Preparation method of ratio-type fluorescent probe and application of ratio-type fluorescent probe in detection of tetracycline antibiotics

Abstract

The invention belongs to the technical field of sensors, and relates to a preparation method and application of a ratio type fluorescent probe. The preparation method comprises the following steps: dissolving fluorescein, zinc acetate and terephthalic acid in DMF (Dimethyl Formamide), carrying out ultrasonic treatment and heating, and after a reactant is cooled, conducting washing and drying to obtain an FSS-coated MOF-5 fluorescent material; and dissolving the FSS-coated MOF-5 fluorescent material in water, adding guanosine monophosphate and europium nitrate under a stirring condition, and conducting washing, centrifuging and drying to obtain the FSS-coated MOF-5-coated GMP / Eu fluorescent probe. The ratio type fluorescent probe prepared by the invention can realize specific detection of tetracycline antibiotics, does not need specific biological materials such as antigens, antibodies or aptamers and the like, and can reduce the time and cost for preparing a specific fluorescent sensor; meanwhile, various color changes can be generated under an ultraviolet lamp, so that semi-quantitative visual detection of tetracycline is realized; and the detection is rapid and accurate, the operation is simple, the probe is not easily influenced by the environment, and the quality and safety of food are guaranteed.

Description

technical field [0001] The invention belongs to the technical field of sensors, and in particular relates to a preparation method and application of a ratio-type fluorescent probe. [0002] technical background [0003] Tetracycline antibiotics, such as tetracycline (TC) and oxytetracycline (OTC), as a broad-spectrum antibiotic with a wide range of uses, have been widely used as feed additives for treating animal disease infections or promoting livestock growth. However, the overuse and misuse of tetracycline antibiotics in livestock and aquaculture has resulted in the accumulation of tetracycline antibiotic residues in milk, meat, eggs, and other foods from animal food sources. Excessive tetracycline antibiotic residues can be passed to humans through the food chain, thereby threatening human health, leading to liver damage, allergic reactions, and bacterial drug resistance. Therefore, it is necessary to quickly and accurately detect the residues of tetracycline antibiotics...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention aims to solve one of the problems; the present invention develops a ratiometric fluorescent probe, which can realize simple, fast, accurate and visual detection of tetracycline antibiotics, and overcome the existing tetracycline antibiotics. Antibiotic detection technology has disadvantages such as complicated operation, long time-consuming, complicated preparation, and unintuitive detection results.

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