Method for extracting and separating stevioside from stevia rebaudiana by using deep eutectic solvents (DES)

A technology of low eutectic solvent and stevia is applied in the preparation of low eutectic solvent and the field of extracting stevia to achieve the effect of shortening the operation process

Inactive Publication Date: 2021-08-03
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] This technology develops a method of extracting stevioside using a low eutectic solvent aqueous solution after a large amount of exploration and research on the composition and performance of DES. There is no similar report at home and abroad. The relevant DES extraction process route (extraction + separation) as attached figure 2 shown

Method used

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  • Method for extracting and separating stevioside from stevia rebaudiana by using deep eutectic solvents (DES)
  • Method for extracting and separating stevioside from stevia rebaudiana by using deep eutectic solvents (DES)
  • Method for extracting and separating stevioside from stevia rebaudiana by using deep eutectic solvents (DES)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1D

[0023] Example 1 DES synthesis (note: this embodiment only uses hydrogen bond acceptor [choline chloride], hydrogen bond donor [ethylene glycol] as reactants, and the rest of the DES synthesis conditions are exactly the same as this example, only the product differences in the hydrogen bond donors in the structure.)

[0024] Add an appropriate amount of choline chloride and ethylene glycol into a one-necked flask for later use, weigh and prepare a mixture of choline chloride: ethylene glycol = 1:2 (molar ratio) for later use; the mixture is stirred at 80°C After 4 hours, a colorless transparent liquid, ie a deep eutectic solvent (DES), was obtained. Store the DES in an environment lower than room temperature for use, and the moisture in it should be within 4%.

Embodiment 2

[0025] Embodiment 2 ethylene glycol DESs are used to extract stevioside

[0026] After the stevia was washed, it was naturally dried and crushed through a 200-mesh sieve. Take 5 grams of stevia and add 20% water content of DES aqueous solution (DES: water = 1:20) g / mL, put it into a 250mL single-necked flask, and put it in a 250mL single-necked flask. Carry out extraction experiment, solid-liquid ratio 1:20 (g / mL), extraction time 180min, after filtering and separating solid-liquid phase, obtain the extract containing stevioside (64.5% RA); Further separation adopts 80% ethanol to carry out alcohol Separation by precipitation to obtain higher content stevioside (81.2% RA), the extraction rate of stevioside per DES is 182mg / g.

Embodiment 3～11D

[0027] Embodiment 3~11DES is used for extracting other embodiments of stevioside

[0028] Examples 7-15 of DES applied to extract stevioside under other different conditions are shown in the table below. The extraction conditions not mentioned in the table are the same as in Example 2.

[0029]

[0030]

[0031] In summary, the present invention provides a preparation method of a low eutectic solvent and its selective adsorption on stevioside. This method uses DES to extract stevioside for the first time, and establishes a new method for selective adsorption and separation of DES.

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Abstract

The invention relates to the technical field of separation of natural active components, and synthesizes five low-eutectic solvents which are used for extracting stevioside. The deep eutectic solvents have better biocompatibility, and the purity of rebaudioside A (RA) is higher than that of a traditional extraction process when stevioside in stevia rebaudiana is extracted. When aqueous solutions of the deep eutectic solvents are used for extracting stevia rebaudiana, stevioside (the extraction capacity can reach 286 mg/g adsorbent) with relatively high RA purity can be extracted. The application of the method simplifies the traditional stevioside extraction process, not only greatly reduces the use amount of an organic solvent, but also has excellent ecological environmental protection and safety. The method provides an efficient new way for extracting and preparing high-purity stevioside from stevia rebaudiana.

Description

technical field [0001] The invention relates to the field of separation of natural active ingredients, specifically, the preparation and application of a low eutectic solvent for extracting stevioside. Background technique [0002] Stevia sugar is a natural high-potency sweetener extracted from the natural plant Stevia rebaudiana. Many countries have approved stevia sugar as a food additive. Its sweetness is 300 times that of sucrose, but its calories are only 1 / 300 of sucrose. At present, as the third generation of sugar to replace sucrose, it has been first used as a sweetener by internationally renowned companies. Natural stevioside is a mixture consisting of several glycoside compounds. Steviosides all contain the same hydrophobic stevioside unit. The difference is that the glycosyl substituents at the C13 and C18 positions are different. The main component is Leba Diglycoside A (Rebaudioside A, RA), stevioside (Stevioside, St), rebaudioside C (Rebaudioside C, RC). The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/256A23L27/10
CPCC07H1/08C07H15/256A23L27/11A23V2002/00A23V2250/262
Inventor 宋航罗英杰邓利红姚舜彭林彩
Owner SICHUAN UNIV
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