Rapid LAMP detection method for watermelon fusarium oxysporum
A watermelon fusarium wilt and detection method technology, applied in the field of watermelon fusarium wilt molecular detection, can solve the problem that the watermelon fusarium wilt LAMP detection technology has not yet been reported and the like
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[0040] 1 Materials and methods
[0041] 1.1 Materials
[0042] Tested strains: including Fusarium oxysporum watermelon specialization type 0, 1, 2 physiological races (FON-0, FON-1, FON-2), Fusarium oxysporum specialization type bitter melon (F.f.sp.momordicae) , Fusarium oxysporum melon specialization (F.f.sp.melonis), Fusarium oxysporum cucumber specialization (F.f.sp.cucumerinum), Fusarium moniliforme (F.moniliforme), F.f.sp. F.f.sp.conglutinans) and Trichoderma harzianum T2-16 (Trichoderma harzianum, T2-16) are both preserved by the Institute of Agricultural Biotechnology, Hunan Academy of Agricultural Sciences. All tested strains were identified through morphological observation and ITS sequence comparison. Zaojia '8424' watermelon seeds (Xinjiang Nongren Seed Technology Co., Ltd.).
[0043] Reagents: 50mmol / L sodium hydroxide (Sinopharm Group Chemical Reagent Co., Ltd.), pH=7.5 Tris-HCl (Beijing Suolaibao Technology Co., Ltd.), 2×LAMP reaction buffer, Bst DNA polymera...
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