Composition with whitening function and containing adipose-derived stem cell exosome
A technology of adipose stem cells and compositions, applied in the biological field, to achieve good preventive effects, delay aging, and anti-oxidative aging effects
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Embodiment 1
[0024] Embodiment 1 component preparation
[0025] Weigh 500g of Yue persimmon, take out the juice of Yue persimmon, add the juice to 20 times the concentration of 40% ethanol, carry out ultrasonic extraction at 300W and 55°C for 2 hours, filter after extraction, and concentrate the filtrate to 50ml to obtain Persimmon extract.
[0026] Rinse 50ml of fat twice, add collagenase to digest for 30 minutes, add fetal bovine serum medium after digestion to stop digestion, remove the upper layer of fat and middle supernatant after centrifugation, then centrifuge for 20 minutes to remove the supernatant, add fetal bovine serum to reconstitute Suspended, filtered through a sieve, transferred to a culture dish for primary culture, and the medium was changed every two or three days. When the cell concentration reached 80%-90% confluence, it was digested with trypsin and subcultured. The subcultured cells were placed in In the culture dish, when the cells are fused to 80-90%, wash the ce...
Embodiment 2
[0035] Embodiment 2 skin melanin experiment
[0036] Select 70 subjects aged 20-30 who are healthy and have no history of allergies to cosmetics. Select the test site at the inner side of the left and right arm of the test subject 5cm from the base of the palm, and the test area is 5×5cm 2 . The subjects were divided into 6 groups, the first five groups were applied with compositions A-E respectively, the sixth group was used as the blank group, and the seventh group was the correction brand nicotinamide essence as the control group. Before the test, the subjects cleaned the inside of the forearm of both hands, The starting value for the test subject's trial area. Then apply the same amount on the same test area in the morning and evening every day for 8 weeks, test the melanin content in the skin on the 7th day, 14th day, 21st day, 28th day, and 60th day, calculate the average value, and list the test results in Table 2 middle.
[0037] Table 2 Skin melanin content test re...
Embodiment 3
[0042] Example 3 In vitro tyrosinase inhibitory ability and antioxidant capacity detection experiment
[0043] Add 500-fold amount of PBS to each composition for dilution, and then test as the final sample.
[0044] Test method for tyrosinase inhibition ability: add 100 μL of sample solutions with different concentrations and 50 μL of 200 U / mL tyrosinase in sequence to a 96-well plate, incubate at 37°C for 10 minutes, and then add 50 μL of 0.05% L-tyrosine acid, mix well, incubate at 37°C for 30min, and measure the absorbance at 490nm. Using the sample solvent as a blank control, 10 parallel experiments were carried out.
[0045] Tyrosinase inhibition rate (%) = (A blank control - A sample) / A blank control × 100%
[0046] DPPH free radical scavenging ability test method: Add 100 μL of 0.25mM DPPH solution and 100 μL of each sample to a 96-well plate in turn, let it stand in the dark for 30 minutes, measure its absorbance at 490 nm, and use the sample solvent as a blank con...
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