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A nutritional composition comprising 6'sl and lnt in combination to improve the gastrointestinal barrier function

A nutritional composition, gastrointestinal barrier technology, applied in the field of intestinal barrier function in children, can solve problems such as infection or allergy

Pending Publication Date: 2021-07-23
SOC DES PROD NESTLE SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This in turn can lead to other problems, such as infections or allergies

Method used

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  • A nutritional composition comprising 6'sl and lnt in combination to improve the gastrointestinal barrier function
  • A nutritional composition comprising 6'sl and lnt in combination to improve the gastrointestinal barrier function
  • A nutritional composition comprising 6'sl and lnt in combination to improve the gastrointestinal barrier function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0188] Table 1 below gives an example of the composition of a nutritional composition (eg infant formula) according to the present invention. This composition is given by way of illustration only.

[0189] Table 1: Composition of the infant formula of Example 1

[0190]

Embodiment 2

[0192] Measuring principle

[0193] Six human milk oligosaccharides (LNnT, LNT, 2'FL, diFL, 6'SL and 3'SL) were screened for the ability to activate the GPR35 receptor in an in vitro assay. Screening was performed by DiscoverX (Fremont, Ca, USA) using the PathHunter β-Arresin assay. This assay uses enzyme fragment complementation with β-galactosidase (β-Gal) as a functional reporter to monitor the activation of GPR35 expressed in cultured cells. The enzyme splits into two inactive complementary parts, EA and PK, which are expressed in cells as fusion proteins. EA is fused to β-arrestin and PK is fused to GPR35. When GPR35 is activated and β-arrestin is recruited to the receptor, PK and EA complementation occurs, restoring β-Gal activity, which is measured using a chemiluminescence detection reagent.

[0194] method

[0195] The GPR35 cell line was expanded from frozen stocks and cultured according to standard procedures. Cells were seeded into white-walled 384-well mi...

Embodiment 3

[0209] The following experiments were performed by digesting LNT and 6'SL and running a GPR35 activation assay such as that of Example 2 using the digested material.

[0210] Digestion of LNT by β-galactosidase

[0211] LNT was digested by β-galactosidase (Megazyme, ref: E-AGLAN) purified from Aspergillus niger using the following protocol: In a 1.5 ml screw-cap tube with an external thread, we mixed 84 mg of LNT in 900 μl of 100 mM Acetate buffer (pH 4.5) and 100 μl of enzyme (400 U). The tube was incubated for 72 h at 55°C with shaking at 450 rpm in a ThermoMix shaker. Enzyme inactivation was accomplished by heat treatment at 100°C for 5 minutes. Inactivated tubes were stored at -20°C until further analysis.

[0212] Digestion of 6'SL by neuraminidase

[0213] The 6′SL was digested by neuraminidase (Megazyme, ref: E-SIALCP) purified from Clostridium perfringens using the following protocol: In a 1.5ml screw-cap tube with an external thread, we mixed 78mg of 6 'SL wa...

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Abstract

The present invention relates to nutritional compositions comprising 6'- sialyllactose (6'SL) and lacto-N-tetraose (LNT) for use in improving the gastrointestinal barrier. In particular, the present invention relates to improving gut barrier function in an infant, a young child or children aged above 3 years to 8 years.

Description

technical field [0001] The present invention relates to a nutritional composition comprising 6'-sialyllactose (6'SL) and lacto-N-tetraose (LNT) for improving the gastrointestinal barrier. In particular, the present invention relates to improving intestinal barrier function in infants, young children or children over 3 years old to 8 years old. Background technique [0002] Breastfeeding is recommended for all infants. However, in some cases, breastfeeding is not sufficient or successful, or the mother does not choose to breastfeed, for some medical reason. Infant formulas have been developed for these conditions. Fortifiers, which enrich breast milk or infant formula with special ingredients, have also been developed. In such cases, it is even more preferred to provide means to improve intestinal barrier function in infants and young children through nutritional intervention. [0003] During postnatal development, the neonatal gut undergoes a maturation process that culm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00A61K31/702A23L33/21A61P1/00
CPCA23L33/21A23L33/40A23V2002/00A23V2200/3202A23V2250/284A61K31/702A61P1/00
Inventor S·达马克F·法塔N·斯普伦格
Owner SOC DES PROD NESTLE SA
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