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A Salmonella phage resistant to antiviral drugs and its application

An antiviral drug and Salmonella technology, applied to viruses/phages, medical raw materials derived from viruses/phages, phages, etc., can solve problems such as ineffective medication, drug resistance of bacteria, and untimely treatment, so as to achieve the goal of treating bacteria with good efficacy. Good tolerance and titer stability

Active Publication Date: 2022-05-31
RECOM QINGDAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a strain of Salmonella phage RDP-SA-20032 resistant to antiviral drugs, aiming to solve the problem of acute onset of salmonella infection in farm poultry and untimely treatment, and drug resistance caused by pathogenic bacteria. Invalid and other issues

Method used

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  • A Salmonella phage resistant to antiviral drugs and its application
  • A Salmonella phage resistant to antiviral drugs and its application
  • A Salmonella phage resistant to antiviral drugs and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] RDP-SA-20032 in the double plate formed plaque diameter of 2mm-4mm.

[0042] The phage proliferation solution was mixed with 60% glycerol in a ratio of 1:1, and then stored in liquid nitrogen.

Embodiment 2

Embodiment 3

[0046] The phage stock solution was dispensed into a 50 ml sterilized conical flask, and the temperature was 0°C, 30°C, 40°C, 50°C, 60°C, 70°C, respectively.

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PUM

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Abstract

The invention discloses a Salmonella phage resistant to antiviral drugs. The preservation number is CGMCC No.21994. After sequencing the whole gene, it is found that it does not have virulence genes and lysogenic genes; the Salmonella phage RDP‑SA‑20032 After being treated at 60°C for 60 minutes, the titer remained at 10 9 pfu / mL, has good tolerance to temperature; in the range of pH 4‑9, cultured at 37 ° C for 1 h, the titer still remains at 10 11 pfu / mL, at pH=12 and pH=2, there are still phages surviving, and the acid-base tolerance range of the phage is wide; the titer stability of the Salmonella phage is good under the coexistence condition of antiviral drugs; The titer is still stable after being dissolved at 4°C for 72 hours, and can withstand higher temperatures. It can be produced by using a conventional spray drying tower to improve production efficiency and reduce production costs.

Description

A Salmonella phage resistant to antiviral drugs and its application technical field The present invention relates to the field of biotechnology, particularly to a Salmonella bacteriophage resistant to antiviral drugs and Its application in pathogenic Salmonella infection of poultry. Background technique Salmonella (Salmonella) is a kind of non-spore straight bacillus parasitic in human and animal intestinal tract, its blood There are more than 2500 serotypes, many of which can be cross-infected between animals and humans, and most serotypes have high Strong pathogenicity has caused great harm to livestock and poultry breeding industry and human beings. Due to the abuse of antibiotics in the poultry industry, Salmonella The resistance of bacteria is getting stronger and stronger, the multi-drug resistant strains are increasing, and depending on the type of antibiotics used, the resistance of Salmonella is increasing. The drug spectrum is also constantly changing. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K35/76A61P31/04A23K10/18A23K50/75A01N63/40A01P1/00C12R1/92
CPCC12N7/00A61K35/76A61P31/04A23K10/18A23K50/75A01N63/40C12N2795/10321C12N2795/10332C12N2795/10331Y02A50/30
Inventor 杜新永张得彦高启兴李先胜马如霞刘玉庆张庆
Owner RECOM QINGDAO BIOTECH CO LTD
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