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Application of diallyl trisulfide to food allergies

A diallyl trisulfide, food allergy technology, applied in the field of medicine, can solve the problems of unresearched and clear food allergy pathogenesis and treatment methods, numerous allergens, complex pathogenic causes, etc., and achieves reduction of specific IgE levels and Mast cell protease levels, alleviation of the decrease in rectal temperature, and reduction in the incidence of diarrhea

Pending Publication Date: 2021-07-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the complex pathogenesis and numerous allergens of food allergies, the pathogenesis and treatment methods of food allergies have not been studied yet. At present, the most effective way is to avoid contact with allergens

Method used

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  • Application of diallyl trisulfide to food allergies
  • Application of diallyl trisulfide to food allergies
  • Application of diallyl trisulfide to food allergies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The establishment of embodiment 1 food allergy model

[0036] All animal experiments were approved by the Animal Ethics Committee of Jiangnan University, and carried out in accordance with national and international animal experiment ethics principles. Female Balb / c mice aged about 5 weeks were randomly divided into normal control group (Control), food allergy group (FA) and DATS group (FA+DATS). There are 6-8 rats in each group, and the feeding conditions are provided by Jiangnan University. The experimental group injected 50 μg of ovalbumin and 1 mg of aluminum hydroxide adjuvant into mice intraperitoneally on the 0th day and the 14th day of the experiment respectively. From the 28th day, 50 mg ovalbumin (Ovalbumin, Ovalbumin, OVA) challenge, 6 consecutive times, the control group was given an equal volume of normal saline. The FA+DATS group was orally administered 10 mg / kg DATS solution every day.

[0037] Within 1 hour of the last 50 mg OVA oral gavage, the recta...

Embodiment 2

[0038] Example 2 DATS relieves food allergy symptoms caused by OVA

[0039] Symptoms characteristic of IgE-mediated experimental food allergy, including anaphylactic diarrhea, decreased rectal temperature, serum levels of OVA-specific IgE and mast cell protease 1 in mice. The establishment method of the animal model is the same as in Example 1, and the severity of food allergy symptoms is analyzed by analyzing the changes of diarrhea and body temperature during the challenge stage of oral gavage. Rats showed a reduced incidence of diarrhea and a smaller decrease in body temperature as figure 1 As shown in (a), the diarrhea rate of mice supplemented with DATS decreased by 25%, and the body temperature change range was reduced to 1.2°C compared with 2.4°C in the model group.

[0040] The levels of OVA-specific IgE and mouse mast cell protease 1 in serum are also indicators of food allergy. Ovalbumin-induced food allergy depends on the production of IgE, thereby activating downs...

Embodiment 3

[0043] Example 3 DATS relieves Th2 cytokine release caused by OVA

[0044] When food allergy occurs, the levels of Th2 cytokines IL-4, IL-5 and IL-13 will increase significantly, and quantitative reverse transcription polymerase chain reaction is used to analyze IL-4, IL-5 and IL-13 in jejunum tissue The mRNA level was detected, and the total RNA was extracted using the TRIzol method according to the instructions. The jejunal tissue samples were first crushed in a high-throughput tissue grinder, and then extracted with chloroform. After centrifugation, the supernatant was aspirated, and an equal volume of isopropanol was added to precipitate RNA. After centrifugation, the supernatant was discarded and washed with 75% ethanol. After two times, dissolve in DEPC water. Reverse transcription was performed using Super Script II reverse transcriptase.

[0045] Use SYBR Green Supermix dye and specific primers (synthesized by Sangon Bioengineering (Shanghai) Co., Ltd.) for PCR analy...

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Abstract

The invention discloses application of diallyl trisulfide to food allergies, and belongs to the technical field of medicine. The invention provides new application of diallyl trisulfide to relief and treatment of food allergies. Animal experiments prove that the diallyl trisulfide can reduce the incidence rate of diarrhea of food allergy mice, relieve the decrease of the rectal temperature, reduce the serum OVA specific IgE level and mast cell protease level, has a relieving effect on jejunum tissue damage, can be used for preparing a medicine, a pharmaceutical composition, a functional food or a formula food for special medical purposes for treating food allergies, and has a wide application prospect.

Description

technical field [0001] The invention relates to the application of diallyl trisulfide in food allergy and belongs to the technical field of medicine. Background technique [0002] Food allergy is an allergic disease caused by an associated adverse immune response. Studies have shown that this disease is mainly mediated by immunoglobulin E, showing gastrointestinal symptoms. IgE food allergy is mainly caused by activation of mast cells by food-specific IgE antibodies. Estimates of the prevalence of food allergy vary by age, geographic location, and study methodology, but the overall conclusion is that the condition is widespread and has been increasing, especially in certain parts of Western countries rate as high as 10%. In 1995, the World Health Organization (WHO) and the United Nations Food and Agriculture Organization (Food and Agriculture Organization of the United Nations, FAO) announced eight categories of common food allergies, including peanuts, soybeans, eggs, mi...

Claims

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Application Information

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IPC IPC(8): A61K31/255A61K45/06A61P37/08A23L33/10
CPCA61K31/255A61K45/06A61P37/08A23L33/10A23V2002/00A61K2300/00A23V2200/304
Inventor 孙嘉潘礼龙刘艳艳
Owner JIANGNAN UNIV
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