Tissue culture propagation method of bryum caespiticium

Abstract

The invention discloses a tissue culture propagation method of bryum caespiticium. According to the tissue culture propagation method of the bryum caespiticium, the sterilization condition of gametophytes is that 75% ethanol, 1% NaClO and 0.01% HgCl2 are used for treating the bryum caespiticium for 30 seconds. The optimal culture conditions of a regeneration system for the bryum caespiticium are that the photoperiod is 12h / d, the pH value is 6, and 1 / 2MS, 3.0% sucrose and 0.1 mg / L 6-BA are adopted. The optimal culture conditions of the regeneration system of the gametophytes of the bryum caespiticium are that the photoperiod is 16h / d, the pH is 6.0, and 1 / 2MS, 3.0% cane sugar, 1.0 mg / L 6-BA and 0.5 mg / L IBA are adopted. According to the tissue culture propagation method of the bryum caespiticium, the disinfection mode and the growth condition which are most suitable for the tissue culture propagation method of bryum caespiticium are screened out, the breeding quantity of the bryum caespiticium is increased, so that a foundation is laid for fully exerting multiple ecological functions of the bryum caespiticium, and an ideal material is provided for vegetation restoration of an ecological system of a drought and degeneration mining area.

Description

technical field [0001] The invention belongs to the technical field of moss tissue culture methods, and in particular relates to a tissue culture and propagation method of moss clumps. Background technique [0002] The Baiyun Obo mining area is located in the dry, cold and windy temperate continental hinterland, with large temperature variations, which is a typical desert steppe climate. The soil in the mining area has rough texture, high sand content, thin soil layer, poor physical structure, severe wind erosion and desertification, scarce plant resources, and a very fragile ecosystem. Due to the year-round mining activities in the Baiyun Obo mining area, the ecological environment of the mining area and its surrounding areas has deteriorated year by year. The survey found that bryophytes are the main dominant plant species in the Baiyun Obo mining area, which can absorb and enrich heavy metals including rare earth elements, which is of great significance for improving the...

AI Technical Summary

Problems solved by technology

However, due to the particularity of the morphological structure of bryophytes, it is very difficult to culture their tissues, and the technological progress of artificial mass propagation is quite slow. There are still many bottlenecks in the current research work, especially the successful establishment of a There are few reports on the asexual reproduction system of moss. The difficulty is that the disinfection conditions of the gametocytes are not easy to grasp, and it is very easy to be infected with bacteria, which leads to the failure of the experiment.

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