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Application of herpetrione extract in preparation of medicine for treating non-alcoholic fatty liver disease

A technology for fatty liver disease and porphyrin, which is applied in the field of traditional Chinese medicine monomer compounds, to achieve the effects of broadening the scope of application, improving fatty lesions, and significantly protecting liver damage

Active Publication Date: 2021-07-16
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the anti-nonalcoholic fatty liver disease activity of the lignan (herpetrione, HPE) in the melon seeds.

Method used

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  • Application of herpetrione extract in preparation of medicine for treating non-alcoholic fatty liver disease
  • Application of herpetrione extract in preparation of medicine for treating non-alcoholic fatty liver disease
  • Application of herpetrione extract in preparation of medicine for treating non-alcoholic fatty liver disease

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Experimental program
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Effect test

Embodiment 1

[0022] The preparation of embodiment 1, porphyrin

[0023] The preparation method of pollyme, the specific steps are as follows: dry and pulverize the pollyme seeds, soak them in ethanol with a volume fraction of 95% for 24 hours, extract by seepage for 5-6 times, combine the extracts, and separate the extracts with petroleum Ether, ethyl acetate and n-butanol organic solvents were extracted successively, each extracted 3 times, respectively combined to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract, which were concentrated under reduced pressure to obtain extracts of different extraction parts; Ethyl acetate extract 192g, mix the sample with silica gel (100-200 mesh) of equal weight, after drying, load the sample by dry method, first use petroleum ether: acetone (volume ratio 50:50) for forward silica gel column chromatography , and then with petroleum ether: acetone (volume ratio 70:30-45:55) through forward silica gel column chromatography, foll...

Embodiment 2

[0025] Example 2. Study on the effect of the compound porphyrin against MCD diet-induced non-alcoholic fatty liver disease in C57BL / 6J mice

[0026] 1. Grouping and administration of animals

[0027] 40 C57BL / 6J mice were raised in an SPF animal laboratory with a temperature of 20°C-25°C and a humidity of 45%-50%. Ventilation was maintained. The animal room was light and dark every 12 hours, and the litter was changed regularly. Drinking water, food ad libitum. After one week of adaptive feeding, they were divided into blank control group, model group (MCD), HPE low-dose group (12.5 mg / kg), HPE middle-dose group (25 mg / kg), HPE high-dose group (50 mg / kg) by random number table method. / kg). The blank control group was fed with MCS feed, and the rest of the groups were fed with MCD feed, free to eat and drink. After 4 consecutive weeks, 2 mice in the blank control group and 2 mice in the model group were randomly selected to be sacrificed, and their livers were removed for r...

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Abstract

The invention discloses application of herpetrione or an extract whose main effective component is herpetrione in preparation of a medicine for treating a non-alcoholic fatty liver disease. A extraction method comprises the following steps: crushing herpetospermum pedunculosum seeds, extracting with 95% ethanol, extracting the extracting solution with ethyl acetate, and concentrating under reduced pressure to obtain extract. Experiments show that herpetrione can remarkably reverse rise of levels of liver function indexes ALT and AST in serum of non-alcoholic fatty liver disease C57BL / 6J mice induced by methionine-choline deficiency diet, serum inflammatory factors IL-6 and IL-1beta, TG in liver tissue and the like, reverse reduction of an HDL index, weight of the mice and a liver index, and improve fat lesion of the liver tissue, and has a significant protective effect on liver damage. The invention broadens application range of herpetrione or herpetospermum pedunculosum seed ethyl acetate extract, and has important application value and economic benefits for the development of protective drugs for liver damage.

Description

technical field [0001] The invention relates to a traditional Chinese medicine monomer compound, in particular to the application of porphyrin or an extract whose main active ingredient is porphyrin in the preparation of a medicine for treating nonalcoholic fatty liver disease. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) refers to a clinical syndrome that excludes definite liver damage factors and hepatic fatty degeneration caused by excessive alcohol consumption, and is also a manifestation of liver metabolic syndrome. The incidence of NAFLD in the general population reaches 20% to 30%, of which 75% to 90% are related to morbid obesity; 3% to 5% may further develop into steatohepatitis, liver cirrhosis and liver cancer. In recent years, the global prevalence of NAFLD has continued to rise, and it has replaced hepatitis B as the No. 1 chronic liver disease in my country, thus attracting more and more attention. The pathogenesis of NAFLD is extrem...

Claims

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Application Information

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IPC IPC(8): A61K31/34A61K36/42A61P1/16A61K131/00
CPCA61K31/34A61K36/42A61P1/16A61K2236/333A61K2236/53
Inventor 陈敏廖志华令狐浪宗伟
Owner SOUTHWEST UNIVERSITY
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