A method for evaluating the effect of NK cells against cytomegalovirus

Abstract

The invention provides a system for evaluating the anti-cytomegalovirus effect of NK cells, the system comprising: a first evaluation subsystem for evaluating the anti-cytomegalovirus function of NK cells; a second evaluation subsystem for evaluating NK cells The function of cells to inhibit the expansion of cytomegalovirus; the third evaluation subsystem is used to evaluate the reinfectivity of cytomegalovirus after being acted on by NK cells; the fourth evaluation subsystem is used to evaluate the clearance of cytomegalovirus by NK cells Function. Through the system provided by the present invention, the anti-cytomegalovirus effect of natural killer cells (NK cells) is scientifically and normatively evaluated, and a scientific and systematic reference is provided for the application of natural killer cells in anti-cytomegalovirus. Accordingly, the system provided by the present invention has broad application prospects.

Description

technical field [0001] The invention belongs to the field of medical and biological detection, in particular to a method for evaluating the anti-cytomegalovirus effect of NK cells. Background technique [0002] Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is an effective and even the only way to cure malignant hematological diseases. Infection is the most common complication after allo-HSCT, accounting for nearly 50% of transplant-related deaths. After Allo-HSCT, the number and function of immune cells have not been restored to normal levels in the early stage, and infection is very prone to occur. Among them, cytomegalovirus (CMV) infection became the main cause of death after Allo-HSCT. The incidence of CMV infection after transplantation is as high as 30-80%, and can cause GVHD and bacterial or fungal infection, increase the risk of transplant-related death, and lead to poor transplant outcomes. The preventive and therapeutic effects of antiviral drug...

AI Technical Summary

Problems solved by technology

However, it is unknown whether NK cells expanded in vitro have anti-CMV effects

[0005] Therefore, in this field, there is a lack of systematic assessment of whether NK cells have an anti-CMV effect; what is more, it is unknown whether NK cells expanded in vitro have an anti-CMV effect

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