Method for detecting breast duct of root tissue of kok-saghyz plant by freezing slide preparation technology

Abstract

The invention discloses a method for detecting a breast duct of a root tissue of a kok-saghyz plant by a freezing slide preparation technology. The method comprises the following steps: selecting a complete plant tissue of the kok-saghyz to be detected, and putting into a stationary liquid; performing vacuum air exhaust on the stationary liquid, wherein the air exhaust time is controlled to be 1-3 min; placing at 4 DEG C for 6-8 hours; taking out the plant tissue in the stationary liquid and performing immersion cleaning for three times, wherein each time is 30 min; the plant tissues are dehydrated step by step through a sucrose solution; after the dehydrated plant tissue adsorbs excessive moisture, embedding the dehydrated plant tissue by using a Biosharp embedding agent, placing the plant tissue at -20 DEG C for 30-45 minutes, slicing the root tissue by using a freezing slicer, adding 10% glycerol for sealing, and solidifying and sealing by using the glycerol for 10 minutes to obtain a slide specimen so as to obtain the distribution condition of the breast ducts in the kok-saghyz root tissue. According to the method, the distribution and quantity conditions of the breast ducts in the kok-saghyz roots can be rapidly detected, the detection period is greatly shortened, and the growth conditions of the breast ducts of the kok-saghyz can be intuitively reflected.

Description

technical field [0001] The invention belongs to the technical field of plant tissue identification, and in particular relates to a method for detecting laticiferous ducts of root tissue of rubber grass plants by means of frozen slice technology. Background technique [0002] Rubber grass, where the mevalonate (MVA) pathway is used to regulate natural rubber biosynthesis, rubber grass is diploid (2n=16) with a relatively simple genome of 1.29 Gb in length containing 46,731 predicted proteins Encoding genes, rubber grass roots contain high-molecular-weight rubber molecules, and its properties are comparable to rubber latex of the Brasilian tree, which can be used as an alternative rubber crop. Rubber grass roots have a high rubber content, ranging from 3% to 28% of their total dry weight, and they are also A new source of inulin (linear β-(2-1)-linked fructans), an important material for the food industry and bioethanol production. Therefore, it can be used as an ideal model ...

AI Technical Summary

Problems solved by technology

But, lack in the prior art the method for making slices to detecting root tissue laticiferous duct of rubber grass plant, therefore need a kind of method of frozen slice technology detection root tissue laticiferous duct of rubber grass plant

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