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Application of Sphondin as effective component in preparation of drugs for treating hepatitis B

A technology for hepatitis B and bovine parsnip, which is applied in the field of biomedicine and can solve problems such as hard-to-yin conversion and hard-to-cure

Inactive Publication Date: 2021-07-06
CHONGQING MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

HBV is difficult to cure, which is closely related to the long-term stable existence of cccDNA. In addition, the envelope protein c

Method used

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  • Application of Sphondin as effective component in preparation of drugs for treating hepatitis B
  • Application of Sphondin as effective component in preparation of drugs for treating hepatitis B
  • Application of Sphondin as effective component in preparation of drugs for treating hepatitis B

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 1. Experimental method

[0064] 1. Cell culture and transfection

[0065] HepG2-NTCP cell line was cultured in DMEM medium containing 10% fetal bovine serum and 2 μg / mL doxycycline; PHH cells were cultured in HM medium; Huh-7 cell line was cultured in DMEM medium containing 10% fetal bovine serum Base. All cells were routinely cultured in a 37°C incubator containing 5% CO2.

[0066] 2.MTT experiment

[0067] Sph was dissolved in DMSO to prepare a 50 mM stock solution. will be 1.5×10 4 Huh-7, PLC / PRF / 5, PHH cells or 2×10 4 HepG2-NTCP cells were seeded in 96-well plates. After 24 hours, the Sph stock solution was diluted with growth medium to obtain 500, 250, 125, 62.5, 31.25, 15.6, 7.8, 3.9, 1.95 μM 9 concentrations; and the no-drug treatment group was used as the control, the pure medium was used as the blank, and 100 μl of drug solution was added to each hole in 3 replicate wells for each concentration. After 72 hours, add 10 μl of MTT reagent, incubate at 37°C ...

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Abstract

The invention belongs to the technical field of biological medicines, and particularly discloses application of Sphondin as an effective component in preparation of drugs for treating hepatitis B. It is found for the first time that Sphondin (Sph for short) can inhibit HBsAg secretion by HBV, inhibit the transcriptional level of HBV RNAs, inhibit the replication level of HBV core DNA, inhibit the transcriptional activity of cccDNA and inhibit the levels of HBV RNAs, HBV DNA, HBV S protein and X protein in liver tissue, has an obvious hepatitis B treatment function, and can be used for preparing novel anti-HBV drugs which can reduce the HBsAg level, inhibit cccDNA transcription, and provide wide application prospects in hepatitis B treatment.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the use of bovine parsnip as an active ingredient in the preparation of hepatitis B therapeutic drugs. Background technique [0002] Hepatitis B Virus (HBV) is a member of the Hepadnaviridae family. HBV infection is closely related to the occurrence and development of acute and chronic hepatitis, liver cirrhosis and liver cancer. According to statistics, about 2 billion people in the world have been infected with HBV, of which about 350 million people are chronically infected, and about 1 million people die every year from serious diseases such as liver failure, liver cirrhosis and primary hepatocellular carcinoma caused by HBV infection . my country is a high-incidence area of ​​hepatitis B. Although the hepatitis B vaccine has entered the national basic immunization range, it cannot completely eliminate HBV. According to the national hepatitis B serum epidemiological surv...

Claims

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Application Information

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IPC IPC(8): A61K31/37A61K45/06A61P1/16A61P31/20
CPCA61K31/37A61K45/06A61P1/16A61P31/20A61K2300/00
Inventor 陈娟黄爱龙任放程胜桃任吉华
Owner CHONGQING MEDICAL UNIVERSITY
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