Application of Sphondin as effective component in preparation of drugs for treating hepatitis B
A technology for hepatitis B and bovine parsnip, which is applied in the field of biomedicine and can solve problems such as hard-to-yin conversion and hard-to-cure
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[0063] 1. Experimental method
[0064] 1. Cell culture and transfection
[0065] HepG2-NTCP cell line was cultured in DMEM medium containing 10% fetal bovine serum and 2 μg / mL doxycycline; PHH cells were cultured in HM medium; Huh-7 cell line was cultured in DMEM medium containing 10% fetal bovine serum Base. All cells were routinely cultured in a 37°C incubator containing 5% CO2.
[0066] 2.MTT experiment
[0067] Sph was dissolved in DMSO to prepare a 50 mM stock solution. will be 1.5×10 4 Huh-7, PLC / PRF / 5, PHH cells or 2×10 4 HepG2-NTCP cells were seeded in 96-well plates. After 24 hours, the Sph stock solution was diluted with growth medium to obtain 500, 250, 125, 62.5, 31.25, 15.6, 7.8, 3.9, 1.95 μM 9 concentrations; and the no-drug treatment group was used as the control, the pure medium was used as the blank, and 100 μl of drug solution was added to each hole in 3 replicate wells for each concentration. After 72 hours, add 10 μl of MTT reagent, incubate at 37°C ...
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