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Method for identifying tea oil based on 3D fluorescence spectrum technology

A fluorescence spectrum technology, tea oil technology, which is applied in the field of identification of adulterated tea oil and camellia oil based on 3D fluorescence spectrum, can solve the problems of inability to detect in real time, high detection cost, cumbersome methylation, etc.

Pending Publication Date: 2021-06-29
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The national standard GBT11765-2018 revised in 2018 is aimed at the detection of fatty acid composition of camellia oleifera by gas chromatography. This method is complicated in sample pretreatment, especially methyl esterification is cumbersome and cannot be detected in real time, and the detection cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Sample pretreatment: Take 20 mL of the tea oil sample to be tested, add an equal volume of dichloromethane and mix evenly, place it in a separatory funnel, use 30% ethanol aqueous solution as the extraction agent for conventional extraction, separate, and concentrate the supernatant to 4mL, as the sample to be tested for 3D fluorescence spectroscopy;

[0019] (2) 3D fluorescence spectrum detection: detection instrument: PDA; fluorescence spectrum parameters are detection excitation wavelength is 380nm, emission wavelength is 430nm, slit width is 4nm, step size is 4nm;

[0020] (3) Test results and judgment: Compared with the 3D fluorescence spectrum of genuine camellia oil, there is no significant difference in the overall fluorescence spectrum morphology, characteristic excitation / emission peaks and fluorescence intensity. Determined as camellia oil.

Embodiment 2

[0022] (1) Sample pretreatment: take 20mL of the tea oil sample to be tested, add 40mL of dichloromethane and mix evenly, place it in a separatory funnel, use 50% ethanol aqueous solution as the extraction agent for conventional extraction, separate, and concentrate the supernatant to 4mL , as a sample to be tested for 3D fluorescence spectroscopy;

[0023] (2) 3D fluorescence spectrum detection: detection instrument: PDA; fluorescence spectrum parameters are detection excitation wavelength of 400 nm, emission wavelength of 450 nm, slit width of 4 nm, and step size of 4 nm;

[0024] (3) Test results and judgment: Compared with the 3D fluorescence spectrum of genuine camellia oil, there is no significant difference in the overall fluorescence spectrum morphology, characteristic excitation / emission peaks and fluorescence intensity. Determined as camellia oil.

Embodiment 3

[0026] (1) Sample pretreatment: Take 20mL of the sample to be tested, add 60mL of dichloromethane and mix evenly, then place it in a separatory funnel; use 60% ethanol aqueous solution as the extraction agent for conventional extraction and separation, and concentrate the supernatant to 4mL, as 3D fluorescence spectrum of the sample to be tested;

[0027] (2) 3D fluorescence spectrum detection: detection instrument: PDA; fluorescence spectrum parameters are detection excitation wavelength of 400 nm, emission wavelength of 450 nm, slit width of 4 nm, and step size of 4 nm;

[0028] (3) Test results and judgment: Compared with the 3D fluorescence spectrum of genuine camellia oil, there is no significant difference in the overall fluorescence spectrum morphology, characteristic excitation / emission peaks and fluorescence intensity. Determined as camellia oil.

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Abstract

The invention discloses a method for identifying tea oil based on a 3D fluorescence spectrum technology. The method comprises the following steps of detecting the three-dimensional fluorescence (3D fluorescence spectrum) of the to-be-detected sample oil subjected to extraction treatment or adsorption treatment under the spectrum conditions that the excitation wavelength is 250-500 nm, the emission wavelength is 300-600 nm, the slit width is 2-10 nm and the step diameter is 2-10 nm by adopting a fluorospectro photometer; if the three-dimensional fluorescence excited and emitted by the tea oil characteristics exists, showing that the tea oil is pure and is not adulterated; and if showing the overall three-dimensional fluorescence spectrum morphology, the characteristic spectrum peak and the fluorescence intensity different from those of the pure tea oil, indicating that the tea oil is doped with other oil products. The identification method disclosed by the invention is simple, high in identification speed, high in identification accuracy and strong in specificity, and provides reliable technical guarantee for the tea oil safety and the advanced food oil industry authentic identification.

Description

technical field [0001] The invention relates to a method for identifying camellia oil, in particular to a method for identifying adulterated camellia oil based on 3D fluorescence spectrum, and belongs to the application field of spectrum technology identification. Background technique [0002] Camellia oil, commonly known as Camellia oleifera seed oil, also known as camellia oil and camellia seed oil, is a pure natural high-grade edible vegetable oil extracted from the mature seeds of common camellia oleifera (Camellia oleifera Abel) in the family Theaceae, with golden or light yellow color and high quality Pure, clear and transparent, with a delicate smell and pure taste. Camellia oil is a unique woody vegetable oil in my country, which has high nutritional value and unique health care function. The fatty acid profile and physical and chemical properties of camellia oil are very similar to olive oil, and its smoke point is higher than that of olive oil, making it more suit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/6458G01N21/6428G01N21/01G01N2021/6439G01N2021/0112
Inventor 邵士俊董树清唐聪邱洪灯
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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