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Cytokinin targeted drug-carrying system, preparation method and application thereof

A drug-loading and targeting technology, applied in the preparation of anti-rheumatoid arthritis drugs, preparation of hemocytin-targeted drug-loading system, field of hemocytin-targeted drug-loading system, can solve the problem of rheumatoid arthritis The effect of long-term treatment of rheumatoid arthritis, rheumatoid arthritis can not be cured, reduce patient tolerance and other problems, to achieve the effect of reducing adverse reactions, reducing the concentration of hemocyclin, and increasing the concentration of hemocyclin

Active Publication Date: 2022-07-22
HUNAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rheumatoid arthritis is currently incurable and requires long-term treatment
However, long-term drug treatment usually produces strong adverse reactions, which reduces the patient's tolerance to drugs and affects the effect of long-term treatment of rheumatoid arthritis

Method used

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  • Cytokinin targeted drug-carrying system, preparation method and application thereof
  • Cytokinin targeted drug-carrying system, preparation method and application thereof
  • Cytokinin targeted drug-carrying system, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Synthesis of Prussian blue nanoparticles:

[0038] Take 0.016 g of potassium ferricyanide and 2 g of PVP, successively add them to 40 mL of 0.01 M HCl, and stir to dissolve for 30 min at room temperature. The yellow clear solution obtained after dissolving was placed in an oil bath at 80° C. for static reaction for 20 hours to obtain crude PB nanomaterials.

[0039] The crude PB nanomaterials were dispensed into 1.5 mL EP tubes, and centrifuged at 13500 rpm for 15 minutes at 4°C. After centrifugation, discard the supernatant, combine the materials in each two EP tubes into one tube, add 1 mL of sterile deionized water, mix thoroughly, centrifuge under the same conditions for 15 minutes, and then add 1 mL of sterile deionized water to each tube. The EP tube was concentrated into 1 tube to obtain refined PB material.

[0040] Take 5 μL of 0.05 mg / mL refined PB material, drop it on the cut silicon wafer, place it in an oven to dry at 45°C, and photograph it with a sca...

Embodiment 2

[0059] Take 20 μL of HA@RFM@PB@SE NPs (0.01mg / mL) solution and drop it on the copper mesh covered with carbon film, put it under infrared lamp and dry it at 60℃, use transmission electron microscope to photograph its shape and RFM film Status of the package. like Figure 5 As shown, the HA@RFM@PB@SE NPs displayed a typical core-shell structure.

[0060] 100 μL each of PB NPs, PB@SE NPs, RFM@PB@SE NPs and HA@RFM@PB@SE NPs were placed in the sample cuvette, and their particle size distribution and Zeta were measured by Zeta potential and nanoparticle size analyzers, respectively. potential. structure as Image 6 , Figure 7 shown. like Image 6 As shown, the peak particle size of PB NPs and PB@SE NPs are both around 80 nm, while the particle size distribution of PB@SE NPs is wider, and there are more large-sized particles, indicating that the particle size of PB nanoparticles does not change after loading SE. large, and some particles have increased. The particle size of...

Embodiment 3

[0062] Whole blood samples of SPF adult SD rats were taken, centrifuged at 3000 rpm for 5 min at 4°C, and washed 5 times with PBS to obtain pure red blood cells. 50 μL of 4% red blood cells (v / v) and 950 μL of PB NPs, PB at different concentrations (12.5 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL, and 200 μg / mL, respectively, dispersant in PBS) The @SE NPs, RFM@PB@SE NPs, and HA@RFM@PB@SE NPs were mixed, and the mixture was allowed to stand at 37 °C for 4 hours. The positive control was mixed with pure water and red blood cells, and the red blood cells were 100% hemolyzed after mixing. The mixture was centrifuged at 3000 rpm for 5 minutes at 4°C, and the absorbance of the supernatant at 540 nm was measured using a UV-Vis spectrophotometer. By formula: Hemolysis(%)=(I / I 0)×100% to calculate the hemolysis rate, in the formula, Hemolysis is the hemolysis rate, I represents the absorbance of the detected sample, I 0 Indicates the absorbance of the positive control (100% hemolysis). ...

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Abstract

The invention discloses a cytosine targeted drug-carrying system, comprising cytosine, Prussian blue nanoparticles, a biomimetic membrane wrapping layer and a hyaluronic acid modified layer. The invention also discloses a preparation method of the targeted drug-carrying system, which includes the following steps: synthesizing Prussian blue nanoparticles; preparing biomimetic membranes; using Prussian blue nanoparticles and cytonin to prepare Prussian blue-loading cytolin nanoparticles; using The biomimetic membrane and the Prussian blue-loaded cytolin nanoparticles are prepared by preparing the biomimetic membrane to encapsulate the Prussian blue-loaded cytolin nanoparticles; hyaluronic acid modification is carried out to obtain the cytosine targeted drug-carrying system of the present invention. The cytosine targeted drug-loading system can accurately deliver cytosine to the lesion site of arthritis, prolong the extraction and retention time of the drug at the site of arthritis, and ensure the safety and effectiveness of the treatment. In addition, the invention also discloses the application of the cytonin targeting drug-carrying system in the preparation of anti-rheumatoid arthritis drugs.

Description

technical field [0001] The invention relates to a targeted drug, in particular to a cytoplasmin targeted drug-carrying system. The invention also relates to a preparation method of a cytosine targeted drug-carrying system and an application of the cytosine targeted drug-carrying system in the preparation of an anti-rheumatoid arthritis drug. Background technique [0002] Blood tube is a commonly used medicinal plant of the Tujia family. Blood tube is sweet, slightly acrid, warm. It has the functions of invigorating blood and promoting blood circulation, expelling wind and removing dampness, and promoting qi and relieving pain. Cytokinin is a triterpenoid pentalactone E (schisanlactone E, SE) extracted from blood vessels. Existing studies believe that SE can inhibit the reproduction and growth of rheumatoid arthritis fibroblast-like synovial cells, and inhibit the The expression of its inflammatory factors has the effect of resisting rheumatoid arthritis (RA). [0003] Rh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K47/61A61K31/366A61K36/57A61K36/79A61P19/02A61P29/00
CPCA61K31/366A61K47/6949A61K47/6901A61K47/61A61K36/79A61K36/57A61P19/02A61P29/00
Inventor 王炜余黄合刘斌蔡雄李斌彭彩云江星明
Owner HUNAN UNIV OF CHINESE MEDICINE
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