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Method for improving sperm function of asthenozoospermia by using exosomes

An exosome and asthenozoospermia technology, applied in the medical field, can solve the problems of lack of very effective treatment methods for asthenozoospermia and limited conventional treatment methods for asthenozoospermia, so as to improve sperm motility and fertility outcomes. Effect

Pending Publication Date: 2021-06-29
NANTONG UNIVERSITY
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Problems solved by technology

[0005] Aiming at the technical problem that the current clinical conventional treatment methods for asthenospermia are limited, and most of the asthenospermia diseases of unknown etiology have no very effective treatment methods, the present invention provides a method for improving asthenospermia by using exosomes. methods of sperm function

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  • Method for improving sperm function of asthenozoospermia by using exosomes
  • Method for improving sperm function of asthenozoospermia by using exosomes
  • Method for improving sperm function of asthenozoospermia by using exosomes

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Embodiment 1

[0020] Step 1. Isolation of normal human seminal exosomes

[0021] The specific separation method is as follows: collect the semen of normal fertile volunteers, centrifuge at 12,000 g for 10 min to remove sperm cells and other impurities; filter the seminal plasma with 0.45 μm and 0.22 μm microporous membranes in turn to remove cell debris and macromolecular aggregation Add 2.5 mL supernatant to 300 μL sucrose cushion solution containing 20 mM Tris / 30% sucrose / deuterium oxide at 100,000 g, 4°C, and ultracentrifuge for 90 min; 25% sucrose / deuterium oxide in a sucrose cushion solution at 100,000 g, 4°C, ultracentrifuged for 5 h; exosomes in 30% and 25% sucrose cushions were mixed and washed once with HS solution (100,000 g, 4°C, ultracentrifuged Centrifuge for 90 min); dissolve the obtained exosomes with HS; finally perform BCA protein quantification, adjust the concentration to 10 times the physiological concentration and store it at -80°C for later use.

[0022] Step 2: Scree...

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Abstract

The invention discloses a method for improving sperm function of asthenozoospermia by using exosomes, and belongs to the technical field of medicine. The method comprises the following three steps: separation of normal human seminal plasma exosomes, screening and pretreatment of asthenospermia sperms, and intervention of the exosomes on the asthenospermia sperms. The seminal plasma exosomes from the human body are used for intervening sperms in vitro, functions of the sperm motility and the like are improved, so that the asthenozoospermia is intervened and treated through simple non-invasive means such as artificial fertilization, and the fertility outcome of a patient is improved.

Description

technical field [0001] The invention belongs to the field of medical technology, and in particular relates to a method for improving sperm function in asthenospermia by using exosomes. Background technique [0002] Male reproductive health has become a focus of close attention in today's human society. According to WHO statistics, the infertility rate of couples of childbearing age in the world is 10-15%, of which, infertility caused by male factors accounts for about half. Sperm quality problems are the main cause of male infertility. Epidemiological surveys have found that weak sperm has become a major factor leading to male infertility, and it is increasing year by year. Asthenospermia refers to a type of infertility disease caused by the forward movement percentage of sperm in semen less than 32% (WHO fifth edition). At the same time, weak sperm not only has reduced forward motility, but also is accompanied by abnormalities in other sperm functions, such as capacitati...

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Application Information

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IPC IPC(8): A61K35/52A61P15/08C12N5/076
CPCA61K35/52A61P15/08C12N5/061C12N2509/00
Inventor 张小宁曾旭辉周阳康爱文宋建
Owner NANTONG UNIVERSITY
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