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Reagent for detecting porcine circovirus type 2e and application thereof

A porcine circovirus and reagent technology, applied in microorganism-based methods, microbial determination/inspection, microorganisms, etc., can solve the problems of long detection cycle and achieve the effect of strong specificity, high sensitivity and good specificity

Pending Publication Date: 2021-06-25
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For genotyping of porcine circovirus type 2, the current common detection is mainly through first-generation DNA sequencing after polymerase chain reaction, but the detection cycle is long

Method used

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  • Reagent for detecting porcine circovirus type 2e and application thereof
  • Reagent for detecting porcine circovirus type 2e and application thereof
  • Reagent for detecting porcine circovirus type 2e and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of reagent that embodiment detects porcine circovirus 2e type

[0036] A preparation method and application of a reagent for detecting porcine circovirus 2e type, the steps are as follows:

[0037] 1. Design and synthesis of fluorescent quantitative PCR primers and probes

[0038] According to the gene sequence of porcine circovirus type 2e (NCBI GenBank accession number is KT795287.1), the primer pair PCV2e-117-F, PCV2e-117-R and TaqMan probe PCV2e were designed to amplify the gene sequence of porcine circovirus type 2e Table 1 shows the nucleotide sequences of -117-probe, PCV2e-81-F, PCV2e-81-R, TaqMan probe PCV2e-81-probe, primer pairs and fluorescent probes.

[0039] By comparing the nucleotide sequences of porcine circovirus type 2e with other type 2 porcine circoviruses, figure 1It is a similarity comparison curve between each genotype and porcine circovirus 2e (PCV2e). It can be seen from the figure that the ORF2 gene of porcine circovirus 2e is found to...

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Abstract

The invention discloses a reagent for detecting porcine circovirus type 2e and application thereof. The reagent comprises a forward primer sequence as shown in SEQ ID No. 1 and a reverse primer sequence as shown in SEQ ID No. 2. According to the reagent, the primers and a fluorescent probe are designed, the primer and probe sequence binding site specificity is good, the porcine circovirus type 2e can be accurately distinguished from other types, and the prepared reagent has characteristics of strong specificity, high sensitivity, time saving and labor saving, and can rapidly perform typing detection on the porcine circovirus type 2e.

Description

technical field [0001] The invention belongs to the technical field of virus detection, and in particular relates to a reagent for detecting porcine circovirus type 2e and an application thereof. Background technique [0002] Porcine circovirus (Porcine circovirus, PCV) is a single-stranded circular DNA virus belonging to the family Circoviridae and the genus Circovirus. According to the difference of antigenicity and pathogenicity, porcine circovirus is divided into porcine circovirus type 1, porcine circovirus type 2 and porcine circovirus type 3. Porcine circovirus type 1 is generally considered non-pathogenic. Porcine circovirus type 3 is thought to co-infect with porcine circovirus type 2. Pigs infected with porcine circovirus type 2 and porcine circovirus type 3 usually develop porcine circovirus-related diseases, mainly including multisystemic wasting syndrome, porcine dermatitis nephrotic syndrome, reproductive failure and porcine respiratory disease syndrome in we...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6851C12R1/93
CPCC12Q1/701C12Q1/6851C12Q2531/113C12Q2561/101Y02A50/30
Inventor 翟少伦吕殿红温肖会翟颀
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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