Method for separating echinococcus granulosus eggs from dog manure and hatching oncosphere in vitro for primary infection animal modeling

Abstract

The invention discloses a method for separating echinococcus granulosus eggs from dog manure and hatching oncosphere in vitro for primary infection animal modeling. According to the technical scheme, the method for preparing the echinococcus granulosus oncosphere comprises the steps that the echinococcus granulosus eggs are obtained through separation, the echinococcus granulosus eggs are hatched with pepsin, shelling is conducted with shelling liquid, purification is conducted with a Percoll culture solution, and the echinococcus granulosus oncosphere is obtained. The Percoll culture solution is formed by mixing 10 * NCTC135, a glutamine solution, gentamicin and Percoll. Experiments prove that by means of the preparation method, the oncosphere which is few in impurity, high in purification degree and high in survival rate can be obtained through hatching, a hepatic echinococcosis primary infection model can be obtained after a mouse is infected with the obtained oncosphere through the hepatic portal vein, and a model and a research basis are provided for the fields of echinococcosis pathogenesis, drug research and development and the like in the follow-up process.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for isolating Echinococcus granulosus eggs from dog feces and incubating hexacarids in vitro for primary infection animal modeling. Background technique [0002] Echinococcosis, commonly known as echinococcosis, is a very harmful zoonotic parasitic disease caused by the larvae of Echinococcus tapeworm (Echinococcus). Echinococcus requires two mammalian species to complete its life cycle. The adult, tapeworm stage parasitizes the small intestine of the final host, the canine; the cyst is the larval stage of Echinococcus, which parasitizes humans, herbivorous livestock, or rodents In the solid organs of animals (intermediate hosts), liver and lung are the main ones. There are two main types of echinococcosis causing human infection: Cystic Echinococcus (CE) caused by Echinococcus granulosus (Eg) and Echinococcus multilocularis (E.m ) caused by Alveolar Echinococcosis (A...

AI Technical Summary

Problems solved by technology

However, the current model of hepatic cystic echinococcosis is often to establish a secondary infection model through liver puncture or hepatic portal vein injection of experimental animals using protocercaria or cysts, which is different from the primary infection that exists in nature. There are many differences, so there is still a certain gap in the application of the experimental data obtained on this model to human beings.

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