Method and kit for simultaneously detecting various bile acids in blood sample
A technology for blood samples and bile acids, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of lack of reference range for healthy people, low method throughput, etc., and achieves the effect of saving time, simple and fast operation, and reliable results.
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[0054] A method for simultaneously detecting multiple bile acids in a blood sample, the steps are as follows:
[0055] (1) Prepare the sample to be tested
[0056] After taking the sample and vortexing for 30s, take 400μl of the suspension sample in a 1.5ml Ep tube, centrifuge at 13000rpm at 4°C for 15min; take 100μL of the supernatant, put it in a 1.5mL Ep tube, add 100μL of methanol to dilute; add 1μL of N-1- Naphthylphthalic acid internal standard solution (0.033mM) was mixed and then filtered with a 0.22μm microporous membrane, and all samples were transferred to a 96-well plate, covered with aluminum foil, and used for detection;
[0057] (2) Ultra-high performance liquid chromatography-mass spectrometry detection
[0058] The sample to be tested enters the chromatographic column for separation through the gradient elution mode, and the reference conditions of liquid chromatography are:
[0059] Chromatographic column: Waters ACQUITYUPLC HSS T3 (1.8μm, 2.1×100mm)
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