A polypeptide combination marker, detection kit and method for early diagnosis of diabetes
A diagnostic kit and marker technology, applied in disease diagnosis, biological testing, measuring devices, etc., can solve the problems of low specificity, lack of sensitivity of markers, etc., and achieve high diagnostic specificity, high sensitivity, and good stability Effect
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Embodiment 1
[0044] The extraction of serum endogenous polypeptides: using ultrafiltration centrifugation. Specific operation process: Take blood from ‑80℃ refrigerator
[0047] Differential polypeptide screening: a total of 163 differential polypeptides were screened. Further select 21 in which the difference fold change is larger
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Embodiment 2
[0054] The extraction of serum endogenous polypeptides: Reference Example 1.
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[0074] Note: L represents a light bid, and M represents a successful bid.
[0076] Stability evaluation: The stability of the target polypeptide was further evaluated, and the results showed that 5 polypeptides were placed in a 4°C refrigerator.
Embodiment 3
[0081] X=4.162+4.364×C(245)+8.905×C(244)-2.725×C(251)-3.326×C(248)-
[0082] Prob (diabetes)=1 / [1+e
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