Heart-type fatty acid binding protein rapid detection card

Abstract

The invention discloses a rapid quantitative test strip for heart-type fatty acid binding protein. The test strip comprises a PVC (polyvinyl chloride) bottom plate, a water absorption pad, a nitrocellulose membrane, a marker pad, a sample pad and a diluent pad, the cellulose membrane is fixed on the bottom plate, one end of the cellulose nitrate membrane is provided with a water absorption pad, and the other end of thecellulose nitrate membrane is provided with a diluent pad; the marker pad is placed on the cellulose membrane and located in front of the diluent pad, and an exposed space of the cellulose membrane is reserved between the marker pad and the diluent pad; a sample pad is arranged on the marker pad; a detection line and a quality control line are sequentially arranged in an interval between the water absorption pad and the marker pad on the cellulose membrane along the direction from the marker pad to the water absorption pad; and a quantitative marking line is arranged between the detection window detection line and the marker pad. According to the invention, a compound for controlling the reaction of a sample solution and a marker bidirectionally laterally flows to a quantitative marking line, so that the immune reaction dose is accurately controlled; and the design of delayed addition of the diluent greatly prolongs the immunoreaction time, and improves the detection sensitivity and precision.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a card for rapid quantitative detection of heart-shaped fatty acid binding protein in immunochromatographic double holes. Background technique [0002] Colloidal gold immunochromatography (GICA) is a rapid immunoassay method developed in the 1970s. It uses nitrocellulose membrane as a carrier, and utilizes the properties of nitrocellulose membrane for protein adsorption and capillary suction. The protein is firmly fixed on the nitrocellulose membrane; and the dripped liquid sample can flow slowly from one side of the membrane to the other under the action of the absorbent pad. When the tested liquid sample contains specific antibodies or antigens, they can immunoreact with the antibodies (or antigens) labeled with colloidal gold to form complexes, and then combine with the corresponding antigens (or antibodies) immobilized on the nitrocellulose membrane to form a d...

AI Technical Summary

Problems solved by technology

During the application process and a large number of tests, a problem was found. Due to the short reaction time between the tested sample and the marker in the marker pad, the reaction between the sample liquid and the labeled antigen or antibody in the marker pad was caused by the attraction of the absorbent pad, resulting in an immune reaction. A gradient distribution is formed, and there is a "dead angle that fails to react", especially when the viscosity difference between the sample matrices is large, the "dead angle" problem is more serious due to the inconsistency of the amount of liquid in the marker pad that participates in the immune reaction. Highlight, resulting in the deviation of the total amount of the tested sample and labeled antigen or antibody permeated into the nitrocellulose membrane, directly affecting the accuracy and stability of the test results

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