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Bacillus velezensis, application thereof and method for controling banana wilt

A technology of Bacillus Velez and banana wilt, applied in the field of biological control, can solve the problems of no growth-promoting effect, insignificant growth-promoting effect, unsatisfactory control effect of bio-control bacteria, etc., and achieve good control of banana fusarium wilt Effect, good growth-promoting effect, effect of simple usage

Active Publication Date: 2021-06-04
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a strain of Bacillus Veles in order to overcome the problems in the prior art that the control effect of bio-control bacteria for the control of banana fusarium wilt is not ideal, and does not have a growth-promoting effect, or the growth-promoting effect is not obvious. , the strain has the characteristics of good control effect on banana fusarium wilt and obvious effect on growth promotion of banana plants

Method used

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  • Bacillus velezensis, application thereof and method for controling banana wilt
  • Bacillus velezensis, application thereof and method for controling banana wilt
  • Bacillus velezensis, application thereof and method for controling banana wilt

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preparation example Construction

[0040] According to a preferred embodiment of the present invention, wherein, the preparation method of the fermentation liquid of Bacillus Velez comprises: cultivating the Bacillus Velez under fermentation conditions to obtain metabolites containing Bacillus Velez of fermentation broth.

[0041] Preferably, the fermentation conditions include: temperature 35-40°C, time 40-60h, 180-220rpm shaking culture.

[0042]Preferably, the concentration of the metabolites of Bacillus Veles in the fermented liquid makes the OD of the fermented liquid 600 The value is 2-2.5, preferably 2.3-2.5.

[0043] In the method provided by the present invention, the fermented liquid can be directly applied to the plants or soil in need, or the metabolites of Bacillus velaisi can be extracted according to the methods of the prior art to make related microbial preparations (such as liquid formulations or solid formulations, etc.) before use. Any existing method for extracting the active ingredient i...

Embodiment 1

[0057] This example is used to illustrate the isolation, identification and preservation of Bacillus velezensis CGMCC NO.21872 provided by the present invention.

[0058] Liquid NA medium formula (1L): peptone 10g, beef extract 3g, sodium chloride 5g, add water 1L, adjust pH to 7.

[0059] The solid NA medium is prepared by adding 15% by weight agar to the liquid medium. The preparation method is to dissolve the ingredients except agar in water, heat to boil, add agar, dissolve and mix well, and use high-temperature steam to sterilize, after taking it out, cool it to about 60°C (it is not hot and the agar in it is not solidified) Pour flat. Use a 9m petri dish, pour about 20mL of the solid NA culture medium into each dish, and form an NA plate after cooling and solidifying.

[0060] (1) Strain isolation and purification

[0061] The rhizosphere soil of healthy banana plants collected from the banana fusarium wilt plot in Yuanjiang County, Yuxi City, Yunnan Province was take...

Embodiment 2

[0076] This example is used to illustrate the inhibitory effect of Bacillus Velez CGMCC NO.21872 provided by the present invention on the pathogenic bacteria of Fusarium wilt of banana.

[0077]In this example, Fusarium oxysporum Cuban specialization No. 4 physiological race tropical type (Foc TR4) was used as the pathogen, and the specific strain used was Foc 15-1 (see literature [3] Lei, Z. , et al., Identification and evaluation of resistance to Fusarium oxysporumf.sp.cubense tropical race 4in Musa acuminata Pahang.Euphytica,2018.214(7):106.).

[0078] In this example, PDA solid medium was used for culturing pathogenic bacteria. The preparation of described PDA solid culture medium and the preparation method of PDA plate are: potato 200g and glucose 20g are added in 1L water, after boiling and mixing uniformly, add agar 15g and mix uniformly, adjust pH to 7, treat to be cooled to after autoclaving. At about 60°C (that is, when the agar is not hot and the agar is not solidi...

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Abstract

The invention relates to the field of biological control, and discloses bacillus velezensis, an application thereof and a method for preventing and / or treating banana wilt. The bacillus velezensis has a relatively strong inhibition effect on fusarium oxysporum f.sp.cubense race 4 (Foc TR4), and can be used for well controling the banana wilt. In addition, the bacillus velezensis also has a very good growth promoting effect on banana plants, and can help the banana plants to grow faster and better. Moreover, the bacillus velezensis is a banana rhizosphere antagonist, cannot generate side effects or pollution problems when being used, and is safer and more environmentally friendly.

Description

technical field [0001] The invention relates to the field of biological control, in particular to a strain of bacillus velei and its application as well as a method for preventing and / or treating banana wilt. Background technique [0002] Banana is a perennial herb and one of the important economic crops in tropical and subtropical regions. my country's banana planting is mainly distributed in Guangdong, Hainan, Guangxi, Fujian, Yunnan and Taiwan, and my country's banana production is huge. At present, bananas are the fourth largest fruit in my country after apples, grapes and citrus. However, the banana planting industry has been plagued by Fusarium wilt of banana in recent years, the pathogen of which is Fusarium oxysporum f.sp. cubenense (FOC) tropical type 4 race (TR4). Banana Fusarium wilt does not show obvious symptoms in the early stage, but the young bananas carry the pathogenic bacteria after infection until they enter the growth period, especially when the buddin...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/07
CPCA01N63/22C12N1/20
Inventor 番华彩徐胜涛曾莉李舒郑泗军何平白亭亭尹可锁李迅东刘立娜郭志祥尚慧
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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