Application of bactericidal composition containing cryptolepine and plant essential oil in prevention and treatment of plant pathogenic fungi
A technology of phyllophylline and plant essential oils, which is applied in the field of natural medicinal chemistry, can solve problems such as the unreported use of plant pathogenic fungi in combination, achieve the effects of reducing pesticide costs, reducing dosage, and improving control effects
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Embodiment 1
[0013] Embodiment 1: a kind of fungicide composition containing phyllophylline and plant essential oil, comprises active component A and active component B (as shown in structural formula 1), and described active component A is phyllophylline, The active component B is plant essential oil.
[0014]
Embodiment 2
[0015] Embodiment 2: Determination of indoor antibacterial activity of phytopathogenic fungi and results
[0016] The phytopathogenic fungi used in this experiment were strains preserved at 4°C in the laboratory, and the mycelial growth method was adopted. All phytopathogenic fungi in the laboratory were grown on potato agar dextrose medium (PDA for short). PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, distilled water 1000mL, natural pH.
[0017] Preparation method of PDA medium: Wash and peel the potatoes, weigh 200g and cut into small pieces, add water and boil until rotten (boil for 20-30 minutes, it can be pierced by a glass rod), filter with four layers of gauze and fill the water in a beaker To 1000mL, add 15-20g of agar according to the needs of the experiment, add 20g of glucose, stir to make it fully dissolved, then divide into Erlenmeyer flasks, sterilize at 121°C for 20 minutes, and cool down for later use.
[0018] experimental method
[0019] ...
Embodiment 3
[0029] Example 3: Indoor activity determination of Rhizoctonia solani mixed with basaltine and active component B
[0030] The mixed synergistic effect of fungicides is calculated according to the Wadley (1967) formula to calculate the synergistic effect coefficient after mixing different agents:
[0031]
[0032] In the formula, a and b respectively represent the proportions of phyllophylline (A) and plant essential oil (B) in the mixture, EC 50 (A), EC 50 (B) represent the actual observed EC 50 value, EC 50 (Exp) represents the theoretical EC of two agents A and B mixed according to a:b 50 value, EC 50 (Obs) is the actual observed EC after mixing the two agents of A and B according to a:b 50 value. SR>1.5 is synergistic; SR<0.5 is antagonistic; SR between 0.5 and 1.5 is additive.
[0033] Table 2 shows the indoor virulence synergistic effect of basaltine and active component B on Rhizoctonia solani.
[0034] Table 2 Indoor virulence synergistic effect of basaltine...
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