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Method for grafting new variety of "Hongyu" camellia oleifera seedlings by using distant variety as stock

A new variety, the technology of camellia oleifera, applied in the field of plant cultivation, can solve the problems that affect the development and utilization process of the new variety of "Hongyu" camellia oleifera, and the development and utilization time is short, so as to improve the efficiency of scientific research and production, good economic benefits, and strong operability Effect

Pending Publication Date: 2021-06-04
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its short development and utilization time, most of the relevant technical parameters are in the stage of experimental demonstration, which affects the development and utilization process of the new variety of "Hongyu" Camellia oleifera

Method used

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  • Method for grafting new variety of "Hongyu" camellia oleifera seedlings by using distant variety as stock
  • Method for grafting new variety of "Hongyu" camellia oleifera seedlings by using distant variety as stock

Examples

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Effect test

Embodiment 1

[0023] The method for grafting seedlings of the new variety "Red Feather" Camellia oleifera using distant species as rootstock comprises the following steps: Step 1, kinship identification; Step 2, selection of rootstock; Step 3, asexual reproduction; Step 4, statistics of survival rate; Fifth, the determination of the rootstock;

[0024] In the above step 1, SLAF-seq technology was used to simplify the genomes of the new varieties of "Hongyu" Camellia oleifera, Luchuan Camellia oleifera, Xianghua Camellia oleifera, Nanrong Camellia oleifera, Small Fruit Camellia oleifera, Common Camellia oleifera, Wantian Honghua Camellia oleifera, and Bobai Daguo Camellia oleifera Sequencing, developing polymorphic SLAF tags, obtaining population SNP sites, based on highly consistent population SNPs, using the neighbor-joining algorithm, systematically analyzed the genetic evolution relationship of the tested germplasm, and constructed its evolutionary tree; the results showed that Lu Chuan ...

Embodiment 2

[0030] The method for grafting seedlings of the new variety "Red Feather" Camellia oleifera using distant species as rootstock comprises the following steps: Step 1, kinship identification; Step 2, selection of rootstock; Step 3, asexual reproduction; Step 4, statistics of survival rate; Fifth, the determination of the rootstock;

[0031] In the above step 1, SLAF-seq technology was used to simplify the genomes of the new varieties of "Hongyu" Camellia oleifera, Luchuan Camellia oleifera, Xianghua Camellia oleifera, Nanrong Camellia oleifera, Small Fruit Camellia oleifera, Common Camellia oleifera, Wantian Honghua Camellia oleifera, and Bobai Daguo Camellia oleifera Sequencing, developing polymorphic SLAF tags, obtaining population SNP sites, based on highly consistent population SNPs, using the neighbor-joining algorithm, systematically analyzed the genetic evolution relationship of the tested germplasm, and constructed its evolutionary tree; the results showed that Lu Chuan ...

Embodiment 3

[0037] The method for grafting seedlings of the new variety "Red Feather" Camellia oleifera using distant species as rootstock comprises the following steps: Step 1, kinship identification; Step 2, selection of rootstock; Step 3, asexual reproduction; Step 4, statistics of survival rate; Fifth, the determination of the rootstock;

[0038] In the above step 1, SLAF-seq technology was used to simplify the genomes of the new varieties of "Hongyu" Camellia oleifera, Luchuan Camellia oleifera, Xianghua Camellia oleifera, Nanrong Camellia oleifera, Small Fruit Camellia oleifera, Common Camellia oleifera, Wantian Honghua Camellia oleifera, and Bobai Daguo Camellia oleifera Sequencing, developing polymorphic SLAF tags, obtaining population SNP sites, based on highly consistent population SNPs, using the neighbor-joining algorithm, systematically analyzed the genetic evolution relationship of the tested germplasm, and constructed its evolutionary tree; the results showed that Lu Chuan ...

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Abstract

The invention discloses a method for grafting a new variety of "Hongyu" camellia oleifera seedlings by using a distant variety as a stock. The method comprises the following steps of 1, identifying a genetic relationship; 2, stock selection; 3, vegetative propagation; 4, counting the survival rate; and 5, determination of stocks. In the first step, a SLAF-seq technology is adopted for performing simplified genome sequencing on the new variety of "Hongyu" camellia oleifera, Luchuan camellia oleifera, fragrant flower camellia oleifera, southern camellia oleifera, small-fruit camellia oleifera, common camellia oleifera, camellia chekiangoleosa and Bobai big-fruit camellia oleifera, polymorphic SLAF tags are developed, population SNP loci are obtained, and based on high-consistency population SNP, a neighbor-joining algorithm is adopted to carry out systematic genetic evolution relationship analysis on test germplasm; and the method is easy to operate, capable of obtaining the rootstock-scion combination with the high grafting survival rate, high in operability and beneficial to popularization, has practical significance in improving the scientific research and production efficiency, and can obtain good economic benefits, social benefits and ecological benefits to a certain extent.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for grafting seedlings of a new variety of Camellia oleifera using distant species as stock. Background technique [0002] The selection and breeding of Camellia oleifera varieties in my country began in the mid-1950s, and more than 20 excellent farm varieties and more than 6,000 excellent trees were screened out successively. 226 excellent clones, 19 excellent families, Five excellent hybrid combinations have laid a solid material foundation for the high-yield cultivation and improved varieties of camellia oleifera in my country. At present, the fine varieties of camellia oleifera in Guangxi are mainly ordinary camellia oleifera, and the variety structure is relatively single, which has greatly affected the healthy and sustainable development of the camellia oleifera industry. How to quickly create more and better new varieties and enrich the diversity of impr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/30A01G17/00
CPCA01G2/30A01G17/005
Inventor 夏莹莹陈国臣刘凯王东雪梁斌蔡娅郝丙青江泽鹏梁国校
Owner GUANGXI FORESTRY RES INST
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