Micrografting method for tetraploid acacia
A technology of tetraploid Robinia pseudoacacia and micro-grafting, which is applied in botany equipment and methods, horticulture, application, etc., can solve the problems that restrict the popularization and application of tetraploid Robinia pseudoacacia, complicated process steps, and long seedling breeding period, and achieve high feeding efficiency. Value and economic value, conducive to breeding and promotion, and the effect of short seedling cycle
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Embodiment 1
[0017] A method for micro-grafting of tetraploid black locust, the method comprises the steps of:
[0018] 1) Obtain seedlings of diploid Robinia pseudoacacia seeds: Select diploid Robinia pseudoacacia seeds with full grains and no disease, soak them in 70°C water for 5 minutes, take them out, soak them in 20°C warm water for 2 days, and sow them on the ground with fine sand Germinate on the seedbed, keep the temperature of the seedbed at 25°C and the humidity at 80-85% to facilitate seed germination. After 7 days, the seeds germinate, and when the seedlings grow to the point where the true leaves are about to unfold, they can be used as seedlings for rootstocks.
[0019] 2) Use diploid black locust seedlings as rootstocks and tetraploid black locust tissue-cultured seedlings as scions, using split grafting method (specifically: take the diploid black locust seedlings out of the sand bed, and cut them from 1cm below the terminal buds) Cut the cutting knife vertically at 1.5cm ...
Embodiment 2
[0022] A method for micro-grafting of tetraploid black locust, the method comprises the steps of:
[0023] 1) Obtain seedlings of diploid Robinia pseudoacacia seeds: Select diploid Robinia pseudoacacia seeds with full granules and no disease, soak them in water at 75°C for 3 minutes, take them out, soak them in warm water at 28°C for 1 day, and sow them on the ground with fine sand Germinate on the seedbed, keep the temperature of the seedbed at 27°C and the humidity at 80-85% to facilitate seed germination. After 5 days, the seeds germinate, and when the seedlings grow to the point where the true leaves are about to unfold, they can be used as seedlings for rootstocks.
[0024] 2) Use diploid black locust seedlings as rootstocks and tetraploid black locust tissue-cultured seedlings as scions, using split grafting method (specifically: take the diploid black locust seedlings out of the sand bed, and cut them from 1cm below the terminal buds) Cut the cutting knife vertically at...
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