Platelet extraction method

Abstract

The invention discloses a platelet extraction method which comprises the following steps: carrying out at least two-stage centrifugal treatment on a whole blood sample subjected to anticoagulation treatment, and extracting platelets from an upper plasma layer obtained after each centrifugal treatment, mixing an upper platelet-deficient plasma layer separated after the last centrifugal treatment with a lower blood cell layer separated after the first centrifugal treatment to prepare a whole blood sample again, performing centrifugal treatment on the newly prepared whole blood sample, extracting platelets from the separated upper platelet-rich plasma layer again to obtain the platelet-rich plasma, and mixing and collecting all extracted platelets. The platelet extraction method disclosed by the invention has the advantages of simplicity in operation, high platelet acquisition rate and high acquisition number of large platelets.

Description

technical field [0001] The invention relates to the technical field of platelet extraction, in particular to a platelet extraction method. Background technique [0002] In recent years, platelet transcriptome detection, as one of tumor liquid biopsy techniques, has certain advantages over other liquid biopsy methods. It is large in number, easy to extract, and contains a large amount of RNA, and platelets can receive external signals to specifically process RNA. Studies have found that platelets play a central role in the systemic and local responses associated with tumor growth. The activation of platelet surface receptors and lipopolysaccharides by external stimuli can induce specific splicing of pre-mRNA in circulating platelets. And platelets are not only the "first responders" of trauma, but also the first responders of tumorigenesis and metastasis, and participate in the process of tumor angiogenesis and metastasis. Platelets may undergo sequence-specific mRNA splicin...

AI Technical Summary

Problems solved by technology

[0003] However, the key technology of platelet transcriptome research is the extraction of platelets. The extraction method of Professor Wurdinger's team is the mainstream platelet extraction method. It is realized by two gradient centrifugation, which is simple and easy to implement. However, although this method is simple, the platelet acquisition rate is low. , and a large number of immature large platelets have not been extracted, while the average volume of platelets is the largest platelet characteristic in tumor patients and normal people. The study of platelets extracted by this method will have certain inherent deficiencies, and a large number of tumor-related molecular signals have not been extracted. can get