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Preparation method of chlorella pyrenoidosa peptide chelated calcium with antioxidant activity

A technology of chlorella pyrenoidosa and antioxidant peptides, which is applied in the fields of peptide preparation methods, chemical instruments and methods, organic chemistry, etc., to achieve the effects of simple preparation methods, easy processes, and improved resource utilization

Pending Publication Date: 2021-05-25
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching, there is no report on the preparation of peptide chelated calcium with chlorella as raw material

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0023] Use the algae residue left by chlorella after oil extraction as raw material, add water according to the mass ratio of material to liquid 1:5, add 3000U / g alkaline protease at the same time, adjust the pH to 8.0, and stir at 45°C and 80r / min for enzymolysis 1h, the process was assisted by 180W, 20kHz ultrasonic treatment; after the enzymolysis was completed, it was centrifuged at 8000r / min for 15min, and the supernatant obtained was the enzymolysis solution of Chlorella pyrenoidosa protein; the enzymolysis solution was ultrafiltered to obtain UF2 and UF3 two peptide fragments, collect the filtrate of the antioxidant peptide, dilute the above antioxidant peptide filtrate to 10mg / mL, and measure 20mL of the diluted solution, according to the mass ratio of calcium salt and antioxidant peptide Add calcium chloride at a ratio of 2:1, chelate for 0.5h under the conditions of pH value 5.0 and temperature 45°C, the process is assisted by 180W, 20KHz ultrasonic treatment, and the...

Embodiment 2

[0025] Use the algae residue left by chlorella after oil extraction as raw material, add water according to the mass ratio of solid to liquid 1:30, and add 5000U / g alkaline protease at the same time, adjust the pH to 10.0, stir and enzymolyze at 65°C and 180r / min 3h, the process was assisted by 300W, 40KHz ultrasonic treatment; after the enzymolysis, centrifuged at 8000r / min for 15min, the supernatant obtained was the enzymolysis solution of Chlorella pyrenoidosa protein; the enzymolysis solution was ultrafiltered to obtain Two peptide fragments, UF2 and UF3, were collected to obtain the filtrate of the antioxidant peptide; the above-mentioned antioxidant peptide filtrate was diluted to 10mg / mL, and 20mL of the diluted solution was measured, according to the mass ratio of calcium salt and antioxidant peptide Add calcium chloride at a ratio of 1:5, chelate for 2 hours at a pH value of 10.0, and a temperature of 90°C. This process is assisted by 300W, 40KHz ultrasonic treatment, ...

Embodiment 3

[0027]Using the remaining algae residue of chlorella after oil extraction as raw material, the calcium salt that chelates with the chlorella peptide is selected as calcium sulfate, and other enzymatic hydrolysis conditions and chelation conditions are the same as in Example 1 to obtain chlorella pyrenoidosa Peptides chelate calcium (UF2-Ca and UF3-Ca). IC of UF2-Ca and UF3-Ca for scavenging DPPH free radical 50 The values ​​are 1.5432±0.132mg / mL and 1.8325±0.025mg / mL respectively; the IC of scavenging ABTS free radical 50 The values ​​are 2.5612±0.135mg / mL and 2.7713±0.158mg / mL respectively; 1.5mg / mL of UF2-Ca and UF3-Ca 2 o 2 The survival rate of Caco-2 cells induced by oxidative damage was increased by 19.03±0.25% and 12.38±0.39%, respectively; the calcium bioavailability of UF2-Ca and UF3-Ca was increased by 23.85±0.53% and 20.76±0.08 relative to calcium sulfate, respectively. %.

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Abstract

The invention discloses a preparation method of chlorella pyrenoidosa peptide chelated calcium with antioxidant and calcium absorption promoting activity, and belongs to the technical field of food / agricultural product processing. The method comprises the following steps: carrying out ultrasonic-assisted enzymolysis on chlorella pyrenoidosa, carrying out ultrafiltration grading, carrying out ultrasonic-assisted chelation, and dialyzing, concentrating and drying the mixture so as to obtain the chlorella pyrenoidosa peptide chelated calcium with the activity of resisting oxidation and promoting calcium absorption. The method is simple in process, short in time consumption, low in energy consumption, convenient to operate, safe, free of pollution and capable of being applied to industrial production on a large scale, residual residues of chlorella obtained after grease extraction are used as raw materials, and the bioavailability of chlorella resources is improved; the chlorella pyrenoidosa peptide chelated calcium prepared by the method has double effects of resisting oxidation and improving the bioavailability of mineral calcium, and has important guiding significance on development of nutrient enrichment products and reasonable diet of three meals a day.

Description

technical field [0001] The invention relates to a preparation method of chlorella pyrenoid peptide chelated calcium with anti-oxidation and calcium absorption-promoting activities, belonging to the technical field of food / agricultural product processing. Background technique [0002] Calcium is an essential mineral element for the human body. It is distributed in various parts of the body and mainly exists in the bones in the form of calcium phosphate, which plays an important role in maintaining the strength of the bones. At the same time, calcium is also the "second messenger" that transmits signals in the body, such as muscle contraction, nerve conduction, blood coagulation, etc., and plays an important role in maintaining the normal operation of human physiological functions. At present, diseases caused by insufficient calcium intake are very common, such as osteoporosis in the elderly and rickets in children, and dietary intake is the only source of calcium storage in t...

Claims

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Application Information

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IPC IPC(8): A23L33/165A23L33/18C12P21/06C07K1/34C07K1/107C07K1/14
CPCA23L33/165A23L33/18C12P21/06C07K1/34C07K1/107C07K1/14A23V2002/00A23V2200/30A23V2250/1578A23V2250/55
Inventor 段玉清连红佳张海晖蔡梅红冯愈钦
Owner JIANGSU UNIV
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