Production method of D-aspartic acid

A technique for producing aspartic acid and its production method, which is applied in the field of amino acid production and can solve the problems of low purity of D-aspartic acid, complex process, and low product yield.

Active Publication Date: 2021-05-18
ANHUI BBCA BIOCHEMICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical method requires asymmetric resolution, the process is complex, and the product D-aspartic acid has low purity and no industrial value
Enzymatic production of D-aspartic acid is a commonly used production method at present, generally using L-aspartic acid-β-decarboxylase fermentation broth for conversion reaction, but in the conversion process, the substrate concentration is low and the product yield is low. The cost is high, and further optimization and improvement are needed to increase the yield of D-aspartic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] Preparation of DL-aspartic acid solid: take deionized water and add DL-aspartic acid by-product of aspartame, add liquid caustic soda to dissolve and make a solution with a concentration of 25-30% by mass, add liquid caustic soda Adjust the pH to 7.5-8.5, add aspartic acid racemase, heat up to 70-80°C for reaction, and end the reaction when the material ratio of the isoelectric crystallization in the central control is 0. After decolorization and filtration, ceramic membrane filtration, the temperature of the isoelectric crystallization is cooled to Below 30°C, centrifuge to separate DL-aspartic acid solid for future use.

[0027] Preparation of the reaction base material DL-aspartic acid solution: take deionized water and add L-aspartic acid, dissolve the liquid caustic soda to make a solution with a concentration of 25% to 30% by mass, add liquid caustic soda to adjust the pH to 7.5~ 8.5, add aspartic acid racemase, heat up to 70-80°C for reaction, end the reaction wh...

Embodiment 1

[0031] The present embodiment provides a kind of production method of D-aspartic acid, comprises steps:

[0032] Take 3000L of fermentation broth containing L-aspartic acid-β decarboxylase, the wet weight of bacteria in the fermentation broth is 35g / L, take 3000L of reaction bottom material DL-aspartic acid solution, bottom material DL-aspartic acid The concentration of DL-aspartic acid in the solution is 0.7g / ml, and the fermentation broth is added to the reaction bottom material, the concentration of DL-aspartic acid in the obtained reaction system is 0.35g / ml, and the reaction system is reacted at 45°C for 2h;

[0033] Then start feeding DL-aspartic acid solid, control the reaction pH not less than 6.0, when the total amount of solid feeding reaches 2500kg, stop feeding solid;

[0034] Then add 3000L reaction base material to the reaction system at one time, the concentration of DL-aspartic acid in the base material DL-aspartic acid solution is 0.7g / ml, and react for 3h;

...

Embodiment 2

[0039] The present embodiment provides a kind of production method of D-aspartic acid, comprises steps:

[0040] Take 3000L of fermentation broth containing L-aspartic acid-β decarboxylase, the wet weight of bacteria in the fermentation broth is 35g / L, take 3000L of reaction bottom material DL-aspartic acid solution, bottom material DL-aspartic acid The concentration of DL-aspartic acid in the solution is 0.6g / ml, the fermentation broth is added to the reaction bottom material, the content of DL-aspartic acid in the obtained reaction system is 0.3g / ml, and the reaction system is reacted at 45°C for 2h ;

[0041] Then start feeding DL-aspartic acid solid, control the reaction pH not less than 6.0, when the total amount of solid feeding reaches 2000kg, stop feeding solid;

[0042] Then add 2000L reaction base material to the reaction system at one time, the concentration of DL-aspartic acid in the base material DL-aspartic acid solution is 0.6g / ml, and react for 2h;

[0043] C...

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Abstract

The invention belongs to the technical field of amino acid production, and particularly discloses a production method of D-aspartic acid. The production method of the D-aspartic acid comprises the following steps of: mixing a reaction base material DL-aspartic acid solution with fermentation liquor containing L-aspartic acid-beta decarboxylase for conversion reaction, and performing conversion in a manner of feeding DL-aspartic acid solid and the DL-aspartic acid solution reaction base material in the reaction process. The concentration of the D-aspartic acid in a conversion liquid system obtained by reaction is high, and the yield and purity of a D-aspartic acid product are remarkably improved.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and in particular relates to a production method of D-aspartic acid. Background technique [0002] D-Aspartic acid is an important chiral compound, which plays an important role in medicine, food, etc., and is a raw material for the synthesis of various chiral drugs. [0003] At present, the preparation of D-aspartic acid mainly includes chemical and enzymatic methods. The chemical method requires asymmetric resolution, the process is complex, and the product D-aspartic acid has low purity and no industrial value. The enzymatic production of D-aspartic acid is a commonly used production method at present. Generally, L-aspartic acid-β-decarboxylase fermentation broth is used for conversion reaction, but in the conversion process, the substrate concentration is low and the product yield is low. The cost is high, and further optimization and improvement are needed to increase the yie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P41/00C12P13/20
CPCC12P41/001C12P13/20
Inventor 曹文文付松崔怀成冯学磊王瑞昌闻龙
Owner ANHUI BBCA BIOCHEMICAL CO LTD
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