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Reagent for detecting concentration of lipoprotein particles and use method thereof

A lipoprotein particle and lipoprotein technology, which is applied in particle suspension analysis, measuring device, suspension and porous material analysis, etc., can solve problems affecting precision and poor precision

Active Publication Date: 2021-05-14
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even seriously affect the precision of LDL-P, IDL-P, VLDL-P and other items
Therefore, whether it is the single detection of lipoprotein particle concentration in patent US9239280B2, or the simultaneous detection of lipoprotein cholesterol and lipoprotein particle concentration in CN110108673A, it shows poor precision and is more susceptible to the influence of high triglyceride samples

Method used

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  • Reagent for detecting concentration of lipoprotein particles and use method thereof
  • Reagent for detecting concentration of lipoprotein particles and use method thereof
  • Reagent for detecting concentration of lipoprotein particles and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]Reagent formulation and testing step

[0038]Take the serum 50 ul, add 1950 ul of dilution (see Table 1 below), mix. Take a 5.0ml Polyallomer snap-in, add 3800 ul of the density solution (see Table 1 below), then slowly add a pre-diluted serum sample 1200UL from the bottom. The centrifuge is placed in the rotor VTI-65.2 rotor and centrifuged with a beckman ultracenter. Parameters: Turn speed = 65000rpm, time (see below), temperature = 23 ° C, acceleration = 6, deceleration = 6.

[0039]Carefully moved the sample after centrifugation to the lipoprotein particle concentration detection instrument for detection. The buffer used for detection is 20 mM phosphate buffer, pH 7.0.

[0040]Table 1. Formulation of different combinations

[0041]

[0042]

[0043]

[0044]The part of the above table did not indicate the concentration, such as KBR, which was used to adjust the density of the diluent, and the solid KBR was added to the density of 1.21 g / ml, NABR, sucrose such as 1.21 g / ml, Nabr, sucrose. N...

Embodiment 2

[0046]Precision experiment

[0047]Take a sample, using this detection system, repeatedly detected 10 times, calculate the precision of the lipoprotein particles concentration, and the results are shown in Table 2, the sample formulation employed is the formulation configured in the example, and the formulation 1 in Table 2 1 The recipe 1 in Table 1 (Component: Diluent KBr, density 1.21 g / ml; Density NaCl, 0.1 mM EDTA, 0.1% ethanol, density 1.05 g / ml; centrifugal time 30 min), the same.

[0048]Table 2 Precision (sample 1, Tg concentration: 1.5mm)

[0049]

[0050]

[0051]

[0052]Table 3 Precision (sample 2, Tg concentration: 2.0mm)

[0053]

[0054]

[0055]

[0056]Table 4 Precision (sample 3, Tg concentration: 3.2mm)

[0057]

[0058]

[0059]

[0060]Table 5 Precision (sample 4, Tg concentration: 4.5mm)

[0061]

[0062]

[0063]

[0064]Table 6 Precision (sample 5, Tg concentration: 6.2mm)

[0065]

[0066]

[0067]It can be seen that this test system detects several different lipoprotein particles concentrations, which have better p...

Embodiment 3

[0069]Linear experiment

[0070]Each project takes a high value sample, diluted with water into different concentration gradients, and detects this test system to verify its linear range. The specific linear ranges of the validated items are HDL-P: 1-80umol / L; LDL-P: 20-6000 nmol / L; LPA-P: 2-300 nmol / L; IDL-P: 1-100 nmol / L; VLDL-P: 2-500 nmo / L. The specific results of the determination of the linear range verification are shown in Table 3.

[0071]Table 7 Linear range verification results

[0072]

[0073]

[0074]

[0075]It can be seen that the linear range of these lipoprotein particles concentrations can meet the indicator requirements, covering the clinical detection, which has better linearity within this linear range.

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Abstract

The invention discloses a reagent for detecting the concentration of lipoprotein particles, the reagent is composed of the following components: a diluent, a density solution and a buffer solution, wherein the density solution contains an alcohol substance, and the concentration of the alcohol substance is 0.1%-50%. The invention belongs to the field of in vitro diagnosis. The reagent for detecting the concentration of the lipoprotein particles provided by the invention not only can be used for independently detecting the concentration of the protein particles, but also can be used together with other instrument reagents for detecting subcomponents of lipoprotein cholesterol at the same time without twice detection, so that the time and the cost are saved, and the reagent has a relatively high commercial value, can be widely applied to clinical examination and scientific research experiments.

Description

Technical field[0001]The present invention belongs to the field of in vitro, and is specifically a reagent that can be used to detect particle concentrations of aliphatic protein components in serum samples.Background technique[0002]It is well known that fat protein can be divided into chyloma (CM), extreme low density lipoprotein (VLDL), intermediate density lipoprotein (IDL), low density lipoprotein (LDL), low density lipoprotein (LDL), low density lipoprotein (Idl) Protein (HDL). Clinically, the cholesterol content of each type of lipoprotein is usually determined for guiding diagnosis. At present, there is not only total cholesterol (T-CH), but also LDL-C, HDL-C. Among them, LDL-C is too high to cause atherosclerosis, commonly known as bad cholesterol, and HDL-C has certain protective effects on blood vessels, commonly known as better cholesterol. But recent research surfaces, whether LDL-C, or HDL-C is heterogeneous, and its clinical significance is not exactly the same. For ex...

Claims

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Application Information

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IPC IPC(8): G01N15/06
CPCG01N15/06G01N15/075G01N33/92G01N2015/0053G01N15/1459G01N15/01
Inventor 邹炳德汪屹贾江花
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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