Neisseria gonorrhoeae integrated nucleic acid detection card box

Abstract

A neisseria gonorrhoeae integrated nucleic acid detection card box comprises a box body with a box cover at the top; a splitting cavity, a first cleaning cavity and a second cleaning cavity are sequentially formed in the box body from top to bottom; the splitting cavity leads to the box cover, and the second cleaning cavity leads to a reaction cavity formed in the bottom of the outer side of the box body; plungers with the plunger hole direction perpendicular to the connecting direction are arranged in the pairwise connecting sections of the splitting cavity, the first cleaning cavity, the second cleaning cavity and the reaction cavity respectively, and hydrophobic liquid sealing materials are arranged in the plunger holes of the plungers. An amplification reaction solution is arranged in the reaction cavity, a soluble material layer is arranged in the reaction cavity, and a primer probe mixed solution of neisseria gonorrhoeae PorA pseudogene is embedded in the soluble material layer. According to the invention, the neisseria gonorrhoeae can be rapidly and integrally subjected to PCR amplification and detection under the condition of relatively low cost, and the problems of poor repeatability, time consumption of a detection method, complicated operation and the like in the prior art are solved.

Description

technical field [0001] The invention relates to an integrated nucleic acid detection cartridge for Neisseria gonorrhoeae, belonging to the technical field of medical detection. Background technique [0002] Neisseria gonorrhoeae (Neisseria gonorrhoeae, NG) is the abbreviation of Neisseria gonorrhoeae, which is the pathogenic bacterium of gonorrhea. Among the Neisseria species, only Neisseria meningitidis and Neisseria gonorrhoeae are pathogenic to humans, and other Neisseria species can cause opportunistic infections in humans under certain conditions. Epidemiological survey results show that gonorrhea has always been the most common sexually transmitted disease and ranks first. The distribution of gonorrhea is global, but mainly in the densely populated developing countries. Gonococcal infection is related to age, gender, race, education level, sexual behavior and body immunity, and is also affected by social, cultural and economic factors. [0003] Gonorrhea is one of t...

AI Technical Summary

Problems solved by technology

It can quickly and integratedly perform PCR amplification and detection of Neisseria gonorrhoeae at a relatively low cost, and solve the problems of poor repeatability of the current technology, time-consuming detection methods, and complicated operations.

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